Comparing HEPES-buffered With Bicarbonate-buffered as Handling Media During the ICSI Procedure on Live Birth Rate

Muhammad Fawzy

Status: Completed

Conditions: Infertility

Treatments: Other: Bicarbonate-buffered handling medium

Study type: Interventional

Funder types: Other

Identifiers

NCT03778866

IbnSina-HEPES

Details and patient eligibility

About

The pH stability during human gamete handling for intracytoplasmic sperm injection (ICSI) is physiologically sound. As the pH change for bicarbonate-buffered medium, scientists assumed whether the use of HEPES or MOPS buffered medium would provide more stable pH, thereby better outcomes. However, the oil use and time frame outside the incubators have not been accounted for. This study is to evaluate the use of HEPES buffered medium compared with the bicarbonate-buffered medium on ICSI outcomes.

Enrollment: 1,057 patients

Sex: Female

Ages: 18 to 40 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• ICSI indicated cycles

Exclusion criteria

• severe medical illness

Trial design

Primary purpose: Other

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Triple Blind

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1,057 participants in 2 patient groups

Bicarbonate-buffered Medium

Experimental group

Treatment:

Other: Bicarbonate-buffered handling medium

HEPES-MOPS-buffered Medium

No Intervention group