Comparison of Alternative Embryo Culture Systems

Bruce Shapiro M.D.

Status

Withdrawn

Conditions

Infertility

Treatments

Other: Enclosed embryo culture

Other: Conventional embryo culture

Study type

Interventional

Funder types

Industry

Identifiers

NCT02028897

SAIRB-13-0040

Details and patient eligibility

About

This study will compare two alternative embryo culture systems. Embryos from each patient will be randomly assigned to one of two systems. One group will be cultured conventionally in oil-covered droplets in dishes. The other group will be assigned to an enclosed system.

Full description

Qualifying and consenting IVF patients will have approximately half of their developing embryos cultured in droplets covered with oil. The other approximate half of their embryos will be cultured in small enclosed containers. Embryo assignments will be at random. Regardless of culture system, all embryos will be cultured from the bipronuclear stage to the blastocyst stage in the same (Global) media, without the usual refreshing of media on day 3.

Sex

Female

Ages

18 to 40 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

Female age 18 to 40, seeking IVF treatment
Ability to understand English (to obtain informed consent)
Use of an oocyte donor, sperm donor, gestational carrier, and/or genetic testing (with blastocyst biopsy only) are allowed.

Exclusion criteria

Diminished ovarian reserve as evidenced by either elevated cycle day 3 follicle stimulating hormone (FSH) level (≥10 IU/L) or low antral follicle count (<8 antral follicles)
Fewer than five (5) bipronuclear oocytes
Day 3 embryo biopsy for any reason
No minors may enroll