Comparison of the Methylation Pattern of DNA in Buccal Swabs From Different Time Points

Universitaire Ziekenhuizen KU Leuven

Status: Terminated

Conditions: DNA Methylation

Treatments: Procedure: Buccal swab

Study type: Observational

Funder types: Other

Identifiers

NCT02297009

S57170

Details and patient eligibility

About

A buccal swab will be taken using a cotton stick by rubbing it against the buccal mucosa. Samples will be taken from the same participant at different timepoints.

DNA will be extracted from the buccal cells on the swabs using a commercial extraction kit and will be quantified by a nano-drop spectrophotometer.

We will determine global and gene-specific methylation and hydroxymethylation alterations by UPLC/MS/MS. Hydrolyzed DNA into individual nucleosides will be analyzed for the quantitative measurement of 5-methylCytosine and 5-hydroxymethylCytosine on Triple Quadrupole UPLC/MS/MS platform. Secondly, specific methylation in the CpG islands of tumor suppressor and promoter genes, and genes involved in the oxidative stress pathways will be assessed by PCR-pyrosequencing.

Enrollment: 20 patients

Sex: All

Ages: 18 to 35 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Caucasian

Exclusion criteria

Trial design

20 participants in 1 patient group

Volunteers

Description:

30 volunteers, half men and half women Buccal swab

Treatment:

Procedure: Buccal swab