Complex Molecular Etiology and Cellular Landscape of Hip Osteoarthritis (OASEQ)

University of Turku

Status: Enrolling

Conditions: Osteoarthritis, Hip; Rheumatoid Arthritis

Treatments: Procedure: Total hip arthroplasty

Study type: Observational

Funder types: Other

Identifiers

NCT05278520

2022OASEQ

Details and patient eligibility

About

The purpose of this study is to cast light on the highly complex etiology and cellular landscape of hip osteoarthritis by utilising single-cell and spatial transcriptomics.

Full description

The specific objectives of this project are:

Using the latest single-cell RNA sequencing (scRNAseq) techniques the investigators aim to A) characterize what kind of cell populations are found in different synovial tissues and blood derived samples of OA patients, B) determine how the cell composition differs between arthritic and corresponding non-arthritic tissues, C) map the transcriptional and regulatory landscape of the cells mentioned in A and B, D) determine what are the key molecular pathways activated in OA. To determine if some of the blood-derived immune cell populations could be used as biomarkers for OA. To map the whole transcriptome of OA and non-arthritic control tissue while keeping the morphological context with spatial transcriptomics technologies. Further differentiation and identification of OA endotypes utilizing the single-cell and spatial data.

The project includes a Rheumatoid sub-study where the main objective is to compare arthritic tissue and peripheral blood constituents between OA and rheumatoid arthritis patients to explore the differences in the disease mechanisms.

Enrollment: 65 estimated patients

Sex: All

Ages: 18 to 74 years old

Volunteers: Accepts Healthy Volunteers

Inclusion and exclusion criteria

Inclusion Criteria for the main (OA) study:

Cases: Adult patients with osteoarthritis in the hip joint and who are going through an elective total hip arthroplasty.

Controls: Non-arthritis adult patients who are going through a trauma-based emergency total hip arthroplasty.

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Inclusion Criteria for the Rheumatoid sub-study:

Adult patients with rheumatoid arthritis in the hip joint and who are going through an elective total hip arthroplasty.

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Exclusion Criteria:

• The body mass index must be below 35
• Age < 18 or > 74
• The OA patients may not have diabetes, rheumatoid arthritis (RA), or metabolic syndrome.

For the Rheumatoid sub-study, the exclusion criteria are the same as above except for the RA.

Trial design

65 participants in 3 patient groups

OA cases

Description:

Twenty-five adult patients who have hip osteoarthritis.

Treatment:

Procedure: Total hip arthroplasty

RA cases

Description:

Twenty-five adult patients who have rheumatoid arthritis in the hip joint.

Treatment:

Procedure: Total hip arthroplasty

Non-arthritic controls

Description:

Fifteen adult patients who go through trauma-based emergency total hip arthroplasty and do not have arthritis.

Treatment:

Procedure: Total hip arthroplasty