Complex Ocular Infection, Optimization of Microbiological Diagnosis (ICODIA)

Assistance Publique - Hôpitaux de Paris

Status: Enrolling

Conditions: Endophthalmitis; Keratitis

Treatments: Diagnostic Test: NGS for endohtalmitis / Multiplex PCR for keratitis

Study type: Observational

Funder types: Other

Identifiers

NCT05432336

APHP220259

2021-A02587-34 (Other Identifier)

Details and patient eligibility

About

The purpose of this study is to evaluate the impact of different technique to optimize the microbiological diagnosis of the COI.

• Metagenomic for the endophtalmitis
• Multiplex polymerase chain reaction for corneal abscesses

Full description

Microbiological diagnosis of complex ocular infection (COI) (i.e: endophtalmitis and corneal abscess) is a current challenge. Indeed, endophtalmitis are often germ-free because a lack of microbiological diagnosis due to small volume to analyze and a complex site to attain. The microbiological etiologies of corneal abscesses are more frequently identified.

Since few years, new molecular tools are developed in infectious diseases to optimizing the microbiological diagnosis. The investigators implemented these techniques in our hospital to optimize the microbiological diagnosis of complex ocular infection (COI). Thus, endophtalmitis benefit, when the volume of the ocular sample is sufficient, of molecular techniques (16s PCR and metagenomic shotgun). Corneal abscesses could shortly benefit of multiplex PCR in order to reduce the time to diagnosis.

The impact and accuracy of these techniques is unknown.

Enrollment: 153 estimated patients

Sex: All

Ages: 18+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Adult patient

• Patient presenting or having presented a clinical suspicion of complex ocular infection requiring a sample for microbiological diagnosis:

  • Corneal abscess requiring hospitalization
  • Any suspicion of endogenous or exogenous endophthalmitis.

• Patient not opposed to participating in the research

Exclusion criteria

• Patient under guardianship or curatorship
• Pregnant women

Trial design

153 participants in 1 patient group

Complex ocular infections (COI)

Description:

All patients with an COI (endophtalmitis or hospitalized keratitis)

Treatment:

Diagnostic Test: NGS for endohtalmitis / Multiplex PCR for keratitis