Comprehensive DNA Methylation Profiling in Crohn's Disease

Jinling Hospital, China

Status: Unknown

Conditions: Illumina Human Methylation 850k BeadChip; Methylation; Crohn Disease

Treatments: Genetic: Genome-wide DNA methylation

Study type: Observational

Funder types: Other

Identifiers

NCT03272152

20170808

Details and patient eligibility

About

Previous studies have indicated that abnormal DNA methylation frequently occurs in the mucosa in Crohn's disease. Comprehensive DNA methylation profiling of the inflamed and non-inflamed ileal mucosa of patients with Crohn's disease was performed. Genome-wide DNA methylation was determined using the Illumina HumanMethylation 850k BeadChip.

Enrollment: 14 estimated patients

Sex: Male

Ages: 18 to 60 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Active Crohn's disease(CDAI score≥ 150)

Exclusion criteria

• No ileal lesions
• Under treatment of immunomodulators, biologic agents or steroids

Trial design

14 participants in 3 patient groups

Inflamed CD group

Description:

Inflamed ileal mucosa was obtained from inflammed lesions of active CD patients

Treatment:

Genetic: Genome-wide DNA methylation

Non-inflamed CD group

Description:

Non-inflamed ileal mucosa was obtained from normal sites of active CD patients

Treatment:

Genetic: Genome-wide DNA methylation

Control group

Description:

Control ileal mucosa was obtained from healthy patients

Treatment:

Genetic: Genome-wide DNA methylation