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CRISPR Delivered Anti-BCMA Car-T Therapy for Relapsed or Refractory Multiple Myeloma

T

Thomas Martin, MD

Status and phase

Begins enrollment this month
Phase 1

Conditions

Multiple Myeloma
Refractory Multiple Myeloma
Recurrent Multiple Myeloma

Treatments

Drug: Fludarabine
Biological: Biospecimen Collection
Procedure: Bone Marrow Biopsy
Behavioral: Quality of Life (QoL) Questionnaires
Drug: Cyclophosphamide
Procedure: Leukapheresis
Procedure: Radiographic imaging
Biological: Chimeric Antigen Receptor T cells (CAR-T) Targeting BCMA

Study type

Interventional

Funder types

Other

Identifiers

NCT07340853
NCI-2025-08425 (Registry Identifier)
25707

Details and patient eligibility

About

This phase Ib trial tests the safety, side effects and best dose of clustered regularly interspaced short palindromic repeats (CRISPR) delivered anti-B-cell maturation antigen (BCMA) chimeric antigen receptor (CAR)-T cells (1XX BCMA CAR-T cells) in treating patients with multiple myeloma that has come back after a period of improvement (relapsed) or that has not responded to previous treatment (refractory). Anti-BCMA CAR-T cell therapy is a type of treatment in which a person's T cells (a type of immune system cell) are changed in the laboratory so they will attack cancer cells. T cells are taken from a patient's blood. Then the gene for a special receptor that binds to a certain protein, such as BCMA, on the patient's cancer cells is added to the T cells in the laboratory by a tool called clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. The special receptor is called a CAR. Large numbers of the CAR-T cells are grown in the laboratory and given to the patient by infusion for treatment of certain cancers. Giving chemotherapy before CAR-T cells may decrease the number of lymphocytes (a type of white blood cells) in the blood and may help the 1XX BCMA CAR-T cells fight the cancer cells. Treatment with 1XX BCMA CAR-T cells may be safe, tolerable, and/or effective in treating patients with relapsed or refractory multiple myeloma (RRMM).

Full description

PRIMARY OBJECTIVES:

Dose Escalation:

I. To evaluate the safety and toxicity of administering Chimeric Antigen Receptor T Cells (CAR-T) cells targeting BCMA to participants with Relapsed or Refractory Multiple Myeloma (RRMM).

II. To determine the maximum tolerated dose (MTD) for anti-BCMA CAR-T cells.

Dose Expansion:

III. Determine whether administering conforming CAR T-cell product targeting BCMA to participants with RRMM increases the overall response rate (ORR) compared with historical data for non-CAR agents per International Myeloma Working Group (IMWG) response criteria.

IV. Determine whether administering conforming CAR T-cell product targeting BCMA to participants with RRMM lowers the Grade 2 or greater neurologic events to <10% in RRMM.

SECONDARY OBJECTIVES:

Dose Expansion:

I. To describe the efficacy of conforming CAR-T cell product targeting BCMA in participants with RRMM.

II. To evaluate the feasibility of manufacturing anti-BCMA CAR T-cells locally and ability to produce adequate quantities of vector positive T-cells.

III. To evaluate the safety and toxicity of conforming CAR-T cell product targeting BCMA to participants with RRMM.

EXPLORATORY OBJECTIVES:

I. To determine the degree and impact of CAR-T persistence following anti-BCMA CAR-T cell infusion, on clinical outcomes and safety.

II. Describe changes in health-related quality of life (HRQoL) using the European Organization for Research and Treatment of Cancer - Quality of Life C30 questionnaire (EORTC-QLQ-C30).

III. To describe the efficacy of CAR-T cells targeting BCMA in participants with relapsed or refractory BCMA+ RRMM who were treated with product that did not meet one or more pre-specified release criteria (non-conforming product cohort).

OUTLINE:

Participants in both cohorts will undergo leukapheresis, receive lymphodepleting chemotherapy and then receive a single infusion of BCMA CAR-T therapy. After completion of study treatment, participants are followed up at 30, 60 and 90 days, 6 and 12 months, and then yearly for up to 15 years.

Enrollment

30 estimated patients

Sex

All

Ages

18+ years old

Volunteers

No Healthy Volunteers

Inclusion criteria

  1. Voluntarily sign informed consent form.

  2. Age ≥18 years.

  3. Eastern Cooperative Oncology Group (ECOG) performance status 0 or 1.

  4. Diagnosis of multiple myeloma (per IMWG criteria) with relapsed or refractory disease and has received at least 3 prior lines of therapy including proteasome inhibitor immunomodulatory therapy, and anti-Cluster of differentiation 38 (CD38) antibody therapy.

  5. Participants may have received BCMA targeted therapy and must be at least 6 months from last BCMA therapy.

  6. Participants must have documented evidence of progressive disease within 12 months of the last line of therapy or be refractory/nonresponsive to their most recent line.

  7. Participants must have measurable disease, defined as at least one of the criteria below:

    • Serum M-protein greater or equal to 0.5 grams per deciliter (g/dL).
    • Urine M-protein greater or equal to 200 milligrams, over a 24-hour period (mg/24 h).
    • Serum free light chain (FLC) assay: involved FLC level of ≥ 100 milligrams per liter (mg/L).
  8. Adequate organ function, defined as:

    • Adequate bone marrow function for apheresis and lymphodepleting chemotherapy.
    • Hgb >8 gm/dl (transfusions allowed).
    • Platelets >50,000/microliter (uL) (in the absence of platelet transfusion within 7 days of apheresis, but transfusion permitted prior to lymphodepleting chemotherapy).
    • Absolute neutrophil count (ANC) > 1000/uL in the absence of growth factor support (filgrastim within 7 days or pegfilgrastim within 14 days of apheresis, but growth factor permitted prior to lymphodepleting chemotherapy). For those patients who have evidence of duffy null, ANC >750/uL is allowed.
    • Absolute lymphocyte count (ALC) >300/uL.
    • Alanine aminotransferase/aspartate aminotransferase (ALT/AST) < 3 x institutional upper limit of normal (ULN) and Total bilirubin < 1.5 milligrams per deciliter (mg/dl) x institutional ULN, except with Gilbert's syndrome.
    • Serum creatinine clearance (CrCl) ≥ 30 milliliter per minute (mL/min) using Cockcroft-Gault formula or as measured with a 24 hour urine collection.
    • Adequate cardiac function, defined as left ventricular ejection fraction (LVEF) > 40% as assessed by echocardiogram or multiple uptake gated acquisition (MUGA) and adequate pulmonary function (measured by room air pulse oximetry ≥ 92%).
  9. Women of childbearing potential must have a negative serum or urine pregnancy test AND agree to use highly effective methods of contraception for 1 year after the last dose of anti-BCMA CAR-T cells.

  10. Males who have partners of childbearing potential must agree to use an effective barrier contraceptive method for 6 months after CAR-T therapy.

Exclusion criteria

  1. Autologous transplant within 12 weeks of planned CAR-T cell infusion.

  2. Prior antitumor therapy as follows, prior to apheresis:

    • Investigational therapy within 14 days, or at least 5 half-lives.
    • Monoclonal antibody therapy within 21 days.
    • Cytotoxic therapy within 14 days.
    • Proteasome inhibitor therapy within 14 days.
    • Immunomodulatory therapy within 14 days.
    • Radiotherapy within 14 days - with the exception that if radiotherapy (XRT) covers <5% of marrow reserve - no rest window needed.
  3. Toxicity from previous anticancer therapy must resolve to baseline levels or to Grade 1 or less except for alopecia, peripheral neuropathy and baseline hematologic toxicity that otherwise meets inclusion.

  4. Active CNS multiple myeloma, plasma cell leukemia, primary AL amyloidosis or POEMS syndrome.

  5. Active other malignancy, other than non-melanoma skin cancer, carcinoma in situ (e.g., cervix, bladder, or breast). Any fully treated malignancies or indolent, clinically insignificant malignancies can be discussed among the study team to determine eligibility.

  6. HIV seropositivity.

  7. Serologic status reflects active hepatitis B or C infection. Participants that are positive for hepatitis B core antibody, hepatitis B surface antigen (HBsAg), or hepatitis C antibody must have a negative polymerase chain reaction (PCR) prior to enrollment. (PCR positive participants will be excluded).

  8. Participants with uncontrolled intercurrent illness including, but not limited to ongoing or active infection, symptomatic congestive heart failure, unstable angina pectoris, uncontrolled cardiac arrhythmia, pulmonary abnormalities, or psychiatric illness/social situations that would limit compliance with study requirements.

  9. Pregnant or breastfeeding women are excluded from this study because CAR-T cell therapy may be associated with the potential for teratogenic or abortifacient effects. Because there is an unknown, but potential risk for adverse events in nursing infants secondary to treatment of the mother with CAR-T cells, breastfeeding should be discontinued. These potential risks may also apply to other agents used in this study.

    NOTE: Women of childbearing potential must have a negative serum or urine pregnancy test.

  10. Participants with currently symptomatic central nervous system (CNS) pathology such as epilepsy, seizure disorders, paresis, aphasia, uncontrolled cerebrovascular disease, severe brain injuries, dementia, and Parkinson's disease OR seizure or stroke within 6 months.

  11. History of autoimmune disease (e.g., rheumatoid arthritis, systemic lupus erythematosus) with requirement of immunosuppressive medication within 6 months.

Trial design

Primary purpose

Treatment

Allocation

Non-Randomized

Interventional model

Sequential Assignment

Masking

None (Open label)

30 participants in 3 patient groups

Dose Escalation Starting Dose: 10 × 10^6 CAR + T cells/ infusion
Experimental group
Description:
Participants undergo leukapheresis and then receive lymphodepleting chemotherapy with fludarabine IV over 30 minutes and cyclophosphamide IV over 60 minutes on days -5, -4, and -3. Participants also receive 10 × 10\^6 BCMA CAR-T cells/ infusion over 5-30 minutes on day 1 in the absence of disease progression or unacceptable toxicity. Additionally, participants undergo PET/CT or MRI at screening and urine and blood sample collection, bone marrow biopsy or aspiration throughout the study.
Treatment:
Procedure: Radiographic imaging
Biological: Chimeric Antigen Receptor T cells (CAR-T) Targeting BCMA
Procedure: Leukapheresis
Drug: Cyclophosphamide
Procedure: Bone Marrow Biopsy
Behavioral: Quality of Life (QoL) Questionnaires
Biological: Biospecimen Collection
Drug: Fludarabine
Dose Escalation: Planned Dose: 30 × 10^6 CAR + T cells/ infusion
Experimental group
Description:
Dependent on the safety profile of the starting dose, participants undergo leukapheresis and then receive lymphodepleting chemotherapy with fludarabine IV over 30 minutes and cyclophosphamide IV over 60 minutes on days -5, -4, and -3. Participants also receive 30 × 10\^6 BCMA CAR-T cells/ infusion over 5-30 minutes on day 1 in the absence of disease progression or unacceptable toxicity. Additionally, participants undergo PET/CT or MRI at screening and urine and blood sample collection, bone marrow biopsy or aspiration throughout the study.
Treatment:
Procedure: Radiographic imaging
Biological: Chimeric Antigen Receptor T cells (CAR-T) Targeting BCMA
Procedure: Leukapheresis
Drug: Cyclophosphamide
Procedure: Bone Marrow Biopsy
Behavioral: Quality of Life (QoL) Questionnaires
Biological: Biospecimen Collection
Drug: Fludarabine
Expansion: MTD of Anti-BCMA CAR-T
Experimental group
Description:
Participants undergo leukapheresis and then receive lymphodepleting chemotherapy with fludarabine IV over 30 minutes and cyclophosphamide IV over 60 minutes on days -5, -4, and -3. Participants also receive the MTD of BCMA CAR-T cells/ infusion established in the dose escalation phase over 5-30 minutes on day 1 in the absence of disease progression or unacceptable toxicity. Additionally, participants undergo PET/CT or MRI at screening and urine and blood sample collection, bone marrow biopsy or aspiration throughout the study.
Treatment:
Procedure: Radiographic imaging
Biological: Chimeric Antigen Receptor T cells (CAR-T) Targeting BCMA
Procedure: Leukapheresis
Drug: Cyclophosphamide
Procedure: Bone Marrow Biopsy
Behavioral: Quality of Life (QoL) Questionnaires
Biological: Biospecimen Collection
Drug: Fludarabine

Trial contacts and locations

1

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Central trial contact

HDFCCC Cancer Immunotherapy Program (CIP)

Data sourced from clinicaltrials.gov

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