Cryopreservation Medium With Apigenin on Post-thaw Human Sperm

Chulalongkorn University

Status: Completed

Conditions: Healthy

Treatments: Dietary Supplement: Apigenin

Study type: Interventional

Funder types: Other

Identifiers

NCT06560216

062267

Details and patient eligibility

About

The goal of this clinical trial is to compare the effect of apigenin supplementation in freezing medium with no apigenin supplementation in freezing medium on post-thaw human sperm motility. It will also evaluate the sperm viability, total antioxidant capacity, and oxidative stress markers. The main questions it aims to answer are:

• Does apigenin supplementation in freezing medium improve the post-thaw sperm motility?
• Does apigenin supplementation in freezing medium improve the post-thaw sperm viability, total antioxidant capacity, and oxidative stress?

Full description

All normal semen samples from participants who give written informed consent and meet the eligibility requirements will be included in the study. Prepared semen samples were divided into 3 groups.

1. Pre-freezing sperm suspension
2. Frozen-thawed control without apigenin supplementation in freezing medium
3. Frozen-thawed sperm suspension with apigenin 0.2 mM supplementation in freezing medium Baseline sperm motility will be measured in pre-freezing sperm suspension. The last 2 groups will be stored in liquid nitrogen for at least one week. After thawing, the sperm suspensions will be evaluated for motility, viability, total antioxidant capacity, and oxidative stress markers.

Enrollment: 57 patients

Sex: Male

Ages: 18 to 45 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Healthy men aged 18-45 years who can receive semen analysis services at the Reproductive Biology Unit, Chulalongkorn Hospital, Thai Red Cross Society
• Normal semen analysis results according to the World Health Organization criteria (Volume ≥ 1.4 mL, Sperm concentration ≥ 16 million/mL, Total motility ≥ 42%, Progressive motility ≥ 30%)
• Giving consent

Exclusion criteria

• A history of accidents, radiation to the testes, or a history of male reproductive system surgery
• Undescended testis
• Varicocele
• A history of taking certain medications that affect sperm production, such as chemotherapy, hormones
• Currently having sexually transmitted disease

Trial design

Primary purpose: Supportive Care

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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57 participants in 2 patient groups

Control

No Intervention group

Description:

Frozen-thawed sperm suspension without apigenin supplementation in freezing medium

Apigenin

Experimental group

Description:

Frozen-thawed sperm suspension with apigenin 0.2 mM supplementation in freezing medium

Treatment:

Dietary Supplement: Apigenin