Cryosurvival of Embryos From Dysmorphic Oocytes

V.K.V. American Hospital, Istanbul

Status: Completed

Conditions: Blastocyst; Cryopreservation

Treatments: Procedure: Cryopreservation, thawing and blastocyst culture

Study type: Observational

Funder types: Other

Identifiers

NCT00521443

AH-05/04

Details and patient eligibility

About

Various morphological abnormalities of human oocytes were reported to detrimentally affect embryo development. We observed development of frozen thawed embryos derived from oocytes with normal or abnormal morphological features to the blastocyst stage. Cytoplasmic abnormalities of the oocytes were found to negatively affect cryosurvival potential of the embryos.

Sex: Female

Ages: 18 to 45 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Human embryos derived from morphologically normal or abnormal mature oocytes that fertilized normally by microinjection procedure.
• Embryos were obtained from couples who refused to freeze their supernumerary embryos after fresh embryo transfer in an assisted reproduction treatment cycle.

Exclusion criteria

• Embryos obtained after microinjection to immature oocytes taht were in vitro matured in culture.
• Embryos obtained from abnormally fertilized zygotes.

Trial design

0 participants in 12 patient groups

Normal

Description:

Embryos derived from morphologically normal MII oocytes

Treatment:

Procedure: Cryopreservation, thawing and blastocyst culture

Irregular Shapes

Description:

Embryos derived from irregularly shaped oocytes.

Treatment:

Procedure: Cryopreservation, thawing and blastocyst culture

Large PVS

Description:

Embryos derived from oocytes with large perivitelline space.

Treatment:

Procedure: Cryopreservation, thawing and blastocyst culture

Dark Zona

Description:

Embryos derived from oocytes with dark zona pellucida

Treatment:

Procedure: Cryopreservation, thawing and blastocyst culture

Dark cytoplasm

Description:

Embryos derived from oocytes with dark cytoplasm

Treatment:

Procedure: Cryopreservation, thawing and blastocyst culture

Vacuolar cytoplasm

Description:

Embryos derived from oocytes with vacuolated cytoplasm

Treatment:

Procedure: Cryopreservation, thawing and blastocyst culture

Central granulation

Description:

Embryos derived from oocytes with centrally granulated cytoplasm

Treatment:

Procedure: Cryopreservation, thawing and blastocyst culture

Double extra

Description:

Embryos derived from oocytes with double extracytoplasmic abnormalities

Treatment:

Procedure: Cryopreservation, thawing and blastocyst culture

Double combined

Description:

Embryos derived from oocytes with any combination of one extracytoplasmic and one cytoplasmic anomaly

Treatment:

Procedure: Cryopreservation, thawing and blastocyst culture

Double cytoplasmic

Description:

Embryos derived from oocytes with any two cytoplasmic anomalies

Treatment:

Procedure: Cryopreservation, thawing and blastocyst culture

Triple extra

Description:

Embryos derived from oocytes with triple extracytoplasmic anomaly

Treatment:

Procedure: Cryopreservation, thawing and blastocyst culture

Triple combined

Description:

Embryos derived from oocytes with triple combined anomalies

Treatment:

Procedure: Cryopreservation, thawing and blastocyst culture