Culture Media Microdroplet Geometry With Different Oil Overlay and Its Effect on Embryonic Development.

Ganin Fertility Center

Status: Completed

Conditions: Infertility; Embryonic Development

Treatments: Other: SPL/LM; Other: GPS/PO; Other: SPL/PO; Other: GPS/LM

Study type: Interventional

Funder types: Other

Identifiers

NCT04485806

MH0002

Details and patient eligibility

About

A prospective randomized study included 1695 MII sibling oocytes collected from 100 patients undergoing ICSI.

Full description

Purpose:

to determine the effect of culture media microdroplet geometry of different culture dishes and oil overlay on the pre implantation embryo development in dry incubators

Method:

We used sibling oocytes splits between two different dishes : GPS (life global, USA) and SPL (life science, Korea) with two different oils : light mineral oil (LM)(Irvine, USA) and paraffin oil (PO)(Vitro life, Sweden). All embryos cultured in dry incubators at the same conditions.

statistical analysis done using SPSS version 23

Enrollment: 100 patients

Sex: Female

Ages: 18 to 37 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Female age is ≤ 37 years old with ≥ 8 MII oocytes
• Males with normal semen parameters (WHO 2010).
• We conducted three experiments all with Siblings oocytes splits and all embryos

Exclusion criteria

• Oocyte or sperm donation.
• Sever male factor.
• Any contradictions to undergoing in vitro fertilization or goandotropin stimulation.

Trial design

Primary purpose: Other

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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100 participants in 4 patient groups

GPS / LM versus SPL/LM

Other group

Description:

to determine the effect of micro droplet geometry in 3D dishes (GPS) or 2D dishes (SPL) in combination with light mineral oil (LM).

Treatment:

Other: SPL/LM

Other: GPS/LM

GPS/PO versus SPL/PO

Other group

Description:

to determine the effect of micro droplet geometry in 3D dishes (GPS) or 2D dishes (SPL) in combination with paraffin oil (PO).

Treatment:

Other: GPS/PO

Other: SPL/PO

GPS/PO versus GPS/LM

Other group

Description:

to determine the effect of oil overlay light mineral(LM) or paraffin oil (PO) in combination with paraffin oil 3D dishes (GPS).

Treatment:

Other: GPS/PO

Other: GPS/LM

SPL/PO versus SPL/LM

Other group

Description:

to determine the effect of oil overlay light mineral(LM) or paraffin oil (PO) in combination with paraffin oil 2D dishes (SPL).

Treatment:

Other: SPL/LM

Other: SPL/PO