CXCL9, CXCL10, CXCL11, and CXCR3 in Dry Eye Syndrome

Chonnam National University

Status: Completed

Conditions: Dry Eye Syndrome

Study type: Observational

Funder types: Other

Identifiers

NCT00991679

I-2007-12-111

Details and patient eligibility

About

The aim of this study is to investigate the expression of CXCL9, CXCL10, CXCL11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome.

Thirty-three patients with dry eye (16 Sjögren's syndrome and 17 non-Sjögren's syndrome patients) and 15 control subjects were recruited. The concentrations of CXCL9, CXCL10, and CXCL11 in tears were measured using enzyme-linked immunosorbent assay. The correlation between chemokine levels and tear film and ocular surface parameters was analyzed. Expression of CXCL9, CXCL10, CXCL11, and CXCR3 in the conjunctiva was evaluated using immunohistochemistry. Flow cytometry was performed to count CXCR3+ cells and CXCR3+CD4+ cells in the conjunctiva.

Enrollment: 48 patients

Sex: All

Ages: 24 to 77 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• clinical diagnosis of dry eye syndrome

Exclusion criteria

• active ocular infection or inflammation not associated with dry eye
• drug toxicity
• contact lens wear
• ocular allergy
• ocular surgery within the last 3 months
• lid or lash abnormalities

Trial design

48 participants in 2 patient groups

Normal control subjects

Description:

Normal control subjects who did not show the clinical symptom and findings of dry eye syndrome.

dry eye patients

Description:

Patients with dry eye syndrome who had symptoms of dry eye for more than 3 months, low tear film break up time (BUT, ≤7 sec), low Schirmer test (\<10 mm), low tear clearance rate (\<8X), and positive fluorescein or rose bengal vital staining (≥3) and were not treated with anti-inflammatory agents such as topical cyclosporine or steroids were included in the study.