DALY II Japan/MB-CART2019.1 for DLBCL

Miltenyi Biotec

Status and phase

Enrolling

Phase 2

Conditions

CAR T Cell Therapy

DLBCL - Diffuse Large B Cell Lymphoma

Treatments

Biological: MB-CART2019.1

Study type

Interventional

Funder types

Industry

Identifiers

NCT07288879

jRCT2033250384 (Other Identifier)

M-2023-413

Details and patient eligibility

About

DALY II Japan is a phase II, multi-center, single arm study to evaluate the efficacy, safety, and pharmacokinetics of zamtocabtagene autoleucel (MB-CART2019.1) in patients with relapsed and/or refractory diffuse large B cell lymphoma (DLBCL) after receiving at least two lines of therapy.

Full description

A prospective, single arm, open label, multi-center, phase II study of autologous T cells engineered against both CD19 and CD20 antigens for subjects with relapsed or refractory DLBCL after receiving at least two lines of therapy. The investigational agent is the MB-CART2019.1 cells. After successful screening, subjects will undergo leukapheresis to collect product for manufacturing. In preparation for the fresh product infusion, subjects will undergo a lymphodepleting regimen with cyclophosphamide and fludarabine. Cell infusion will be administered intravenously at a dose of 2.5 x 10^6 CAR+ cells/kg body weight. Subjects will be followed for up to 2 years, for efficacy and safety outcomes as well as health-related quality of life (HRQoL). Additional long-term follow-up will be conducted for participants under a separate long-term follow-up protocol.

Enrollment

31 estimated patients

Sex

All

Ages

18+ years old

Volunteers

No Healthy Volunteers

Inclusion criteria

Histologically confirmed DLBCL or associated subtype, defined by WHO 2016 classification:
- DLBCL not otherwise specified (NOS)
- High-grade B-cell lymphoma with MYC and BCL2 and/or BCL6 rearrangements
- High-grade B-cell lymphoma, NOS
- Primary mediastinal (thymic) large B-cell lymphoma
- Transformed lymphoma (e.g. transformed follicular or marginal zone lymphoma, follicular lymphoma Grade 3B)
Relapsed or refractory disease after 2 or more lines of chemotherapy including rituximab and anthracycline and either having failed autologous stem cell transplant (ASCT), or being ineligible for or not consenting to ASCT 2.1 Chemotherapy-refractory disease is defined as one of the following:
- No response to last line of therapy:
  - Progressive disease (PD) as best response to most recent therapy regimen
  - Stable disease (SD) as best response to most recent therapy with duration no longer than 6 months from last dose of therapy OR
- Relapsed or persistent disease after prior ASCT for lymphoma
  - Disease progression or relapse less than or equal to 24 months of ASCT
  - If salvage therapy is given post-ASCT, the individual must have had no response to or relapsed after the last line of therapy 2.2 Disease relapse in subjects without prior ASCT is defined as relapse of disease in ≤ 12 months after the last dose of most recent therapy regimen 2.3 Ineligible for ASCT is defined as meeting one of the following criteria:
- Chemotherapy-refractory disease after salvage therapy
- Disease progression or relapse ≤ 12 months after salvage therapy
- Intolerance to salvage therapy
In addition, all subjects must have:
Age ≥18 years
Eastern Cooperative Oncology Group (ECOG) performance status that is either 0 or 1 at screening. ECOG performance status of 2 at screen is allowed if the decrease in performance status is due to DLBCL
Measurable disease according to Lugano 2014 criteria for assessing fluorodeoxyglucose-positron emission tomography (FDG-PET)/computer tomography (CT) in lymphoma (Cheson et al, 2014)
CD19 or CD20 antigen expression on tumor is not required after the most recent chemoimmunotherapy; however, 6.1 Subject must have at least 20 unstained slides of tissue available prior to MB-CART2019.1 infusion 6.2 If archival tissue is not available, subject must be willing to undergo attempted repeat biopsy
No clinical suspicion of central nervous system (CNS) lymphoma
If the subject has history of CNS disease, then he/she must 8.1. Have no signs or symptoms of CNS disease 8.2. Have no active disease on magnetic resonance imaging (MRI) 8.3. Have no large cell lymphoma present in cerebral spinal fluid (CSF) on cytospin preparation and flow cytometry, regardless of the number of white blood cells (WBCs)
If the subject has history of cerebral vascular accident (CVA) 9.1. The CVA event must be greater than 12 months prior to leukapheresis 9.2. Any neurological deficits must be stable
An estimated creatinine clearance by Cockcroft-Gault Equation (eGFR) > 60mL/min
Cardiac ejection fraction (EF) ≥ 45% as determined by an echocardiogram (ECHO)
Resting O2 saturation >90% on room air
Serum alanine aminotransferase (ALT) / aspartate aminotransferase (AST) <5 times the Upper Limit of Normal (ULN) for age
Total bilirubin <1.5 mg/dl, except in individuals with Gilbert's syndrome
Absolute neutrophil count (ANC) > 1000/μL
Absolute lymphocyte count > 100/μL
Platelet count > 50,000/μL
Estimated life expectancy of more than 3 months other than primary disease
Subjects of childbearing or child fathering potential must be willing to practice birth control from the time of enrollment on this study until the follow-up period of the study

Exclusion criteria

Primary CNS lymphoma
Richter's transformed DLBCL arising from chronic lymphocytic leukemia (CLL)
Unable to give informed consent
Known history of infection with human immunodeficiency virus (HIV) or active hepatitis B (HBsAg positive), unless confirmed to be polymerase chain reaction (PCR) negative; antiviral prophylaxis is required as recommended in the Japanese guidelines for Hepatitis B treatment if HBsAg negative and anti-HBc positive
Known history of infection with hepatitis C virus (anti-HCV positive) unless viral load is undetectable per quantitative PCR and/or nucleic acid testing
Known history of active seizure or presence of seizure activities or on active anti-seizure medications within the prior 12 months
Known history of CVA within prior 12 months
Known history or presence of autoimmune CNS disease, such as multiple sclerosis, optic neuritis or other immunologic or inflammatory disease
Presence of active CNS disorder that, in the judgment of the investigator, may impair the ability to evaluate neurotoxicity
Active systemic fungal, viral or bacterial infection
Pregnant or breast-feeding woman
Previous or concurrent malignancy with the following exceptions:
- Adequately treated basal cell or squamous cell carcinoma (adequate wound healing is required prior to study entry)
- In situ carcinoma of the cervix or breast, treated curatively and without evidence of recurrence for at least 2 years prior to the study
- Adequately treated breast or prostate carcinoma on hormonal therapies such as Lupron or tamoxifen and in clinical remission of ≥ 2 years
- A primary malignancy which has been completely resected / treated with curative intent and in complete remission of ≥ 2 years
History of non-neurologic autoimmune disease (e.g. Crohn's disease, rheumatoid arthritis, systemic lupus erythematosus) requiring systemic immunosuppressive or systemic disease modifying agents within the last 2 years
Medical condition requiring prolonged use of systemic corticosteroids equivalent to Prednisone >10 mg/day
History of myocardial infarction, cardiac angioplasty or stenting, unstable angina, or other clinically significant cardiac disease within 6 months of enrollment
Concurrent radiotherapy (allow up to time of leukapheresis)
Baseline dementia that would interfere with therapy or monitoring, determined using Immune Effector Cell-Associated Encephalopathy (ICE) Assessment at baseline. (Appendix 6, Section 13.6)
History of severe immediate hypersensitivity reaction to any of the agents used in this study
Refusal to participate in additional lentiviral gene therapy LTFU protocol
Prior CAR T cell therapy for any indication
Prior allogeneic stem cell transplant for any indication.
Prior bispecific antibodies for cancer therapy
Prior T cell receptor-engineered T cell therapy
Prior anti CD 19 immunotherapy
Hypersensitivity against any drug including MB-CART2019.1 (and the constituents used in the production, ingredients/impurities, including bovine and rodent-derived components), that is scheduled or likely to be given during trial participation, e.g. as part of the mandatory preparative chemotherapy or rescue medication/salvage therapies for treatment related toxicities.