Demodex Density, Immune Response and Oxidative Stress in Rosacea Patients

Istanbul Training and Research Hospital

Status: Unknown

Conditions: Rosacea

Treatments: Device: Reflectance confocal microscopy; Device: Sebumeter; Device: Video dermoscopy; Other: Obtaining a blood sample

Study type: Observational

Funder types: Other

Identifiers

NCT02556827

71306642-050.01.04

Details and patient eligibility

About

Aim of study is to determine reactive oxygen products and antioxidant capacity with rosacea patients; to measure metalloproteinase level and inflammatory markers of immune response and investigate their corelation with demodex infestation and also investigate the role of photoaging and sebum secretion in rosacea.

Full description

Rosacea is a common and chronic inflammatory disease characterized by facial erythema, papule, pustule and telangiectasia. Ultraviolet, Demodex mite, oxidative stress and antioxidant system disorders are emphasized in increased inflammation seen in rosacea.

The aim of our study was to evaluate the status of oxidative stress, matrix metalloproteinase (MMP) and inflammatory markers in systemic circulation, their relationship with demodex density and investigate the role of photoaging and sebum secretion in rosacea.

Forty patients with rosacea and age, gender and skin phenotype matched 40 healthy volunteers were enrolled in the study. Reflectance confocal microscopy (RCM) was used to calculate facial demodex density. The number of demodex, follicle, the number of mite per follicle, the number of infested follicle and the number of mite per infested follicle were calculated with RCM.

Photoaging severity and facial sebum levels were also assessed. Total oxidant capacity (TOC), total antioxidant capacity (TAC), oxidative stress index (OSI), paraoxonase-1 (PON-1), aryl esterase (ARES), myeloperoxidase (MPO), tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), matrix metalloproteinase-1 (MMP-1) and matrix metalloproteinase-9 (MMP-9) levels in venous blood were measured.

Enrollment: 80 estimated patients

Sex: All

Ages: 18 to 70 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Between the ages of 18-70
• No systemic illness
• No other inflammatory dermatoses except for rosacea
• No smoking
• Not using any topical, systemic treatment and sunblock at least a month for rosacea or other condition

Exclusion criteria

• Systemic illness
• Other inflammatory dermatoses except for rosacea
• Smoking
• Using any topical, systemic treatment and sunblock for rosacea or other condition
• Type 4 rosacea patient (only ocular involvement)

Trial design

80 participants in 2 patient groups

H, Rosacea patients

Description:

Rosacea patients who were between the ages of 18-70, having no systemic illness and who were not smoking. Obtaining a blood sample; TOC, TAC, OSI, PON-1, ARES, MPO, TNF-α, IL-1β, MMP-1 and MMP-9 levels in venous blood were measured Reflectance confocal microscopy; 1mm2-sized 10 images were taken from right cheek and forehead; the number of demodex, follicle, the number of mite per follicle, the number of infested follicle and the number of mite per infested follicle were calculated with RCM. And photoaging severity were also assessed by using RCM. Sebum rate at forehead and right cheek were evaluated with sebumeter. Dermoscopic photoaging scale were assessed by using video dermoscopy.

Treatment:

Device: Sebumeter

Device: Reflectance confocal microscopy

Device: Video dermoscopy

Other: Obtaining a blood sample

K, Healthy volunteers

Description:

Healthy volunteers who were compatible in terms of age, sex and skin phenotype, having no systemic illness and who were not smoking. Obtaining a blood sample; TOC, TAC, OSI, PON-1, ARES, MPO, TNF-α, IL-1β, MMP-1 and MMP-9 levels in venous blood were measured Reflectance confocal microscopy; 1mm2-sized 10 images were taken from right cheek and forehead; the number of demodex, follicle, the number of mite per follicle, the number of infested follicle and the number of mite per infested follicle were calculated with RCM. And photoaging severity were also assessed by using RCM. Sebum rate at forehead and right cheek were evaluated with sebumeter. Dermoscopic photoaging scale were assessed by using video dermoscopy.

Treatment:

Device: Sebumeter

Device: Reflectance confocal microscopy

Device: Video dermoscopy

Other: Obtaining a blood sample