Demonstration of Carbonyl Stress on Hair Follicles

Pierre Fabre

Status

Completed

Conditions

Effluvium; Telogen

Treatments

Other: Hair follicles sampling

Study type

Interventional

Funder types

Industry

Identifiers

NCT03414476

STRESSCARBONYL1

Details and patient eligibility

About

In this study, the investigator will try to highlight the presence of carbonyl compounds in the hair follicles of women with telogen effluvium (ET) linked to stress of various origins (fatigue, infectious diseases, emotional stress ...).

Full description

At the cutaneous level, oxidative stress induces the formation of carbonyl compounds which, by binding to proteins, produce nonfunctional adducts inducing cutaneous aging. Is this mechanism, called "Carbonyl stress", also present in the scalp and can it be one of the mechanisms involved in the occurrence of reactive hair loss ? To demonstrate this, samples of hair follicles are made. The proteins are extracted from the samples and then assayed. The oxidized (carbonyl) proteins are labeled with specific fluorescent probes.

Enrollment

40 patients

Sex

Female

Ages

18 to 40 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

Criteria related to the Population :

Women aged from 18 to 40 ans (included)
Phototype I à IV included, according to Fitzpatrick Classification
Hair length 5 cm on the sampling area (centro-parietal part)
Last shampoo at least 2 days before the inclusion visit, without application of styling product, care or water between this last shampoo and the inclusion
Informed consent signature

Criteria related to the studied condition:

ET group: hair loss related to various etiologies evaluated by a questionnaire according to the investigator's opinion and positive pull test (at least 3 hair collected per lock of 50 to 60 hair) AND
Control group : no hair loss, evaluated by the questionnaire and negative pull test (less than 3 hair collected per lock of 50 to 60 hair)

Exclusion criteria

Criteria related to Population :

Menopause
Pregnancy, breastfeeding, childbirth for less than 6 months

Criteria related to Pathology :

For both groups:

Alopecia causing hair loss on the upper part of the scalp
Dermatological pathology or evolving cutaneous lesion in the scalp
Control group only:
Chronic unstabilized condition
Acute evolving pathology

Criteria related to theTreatments :

For both groups:

Use for at least 1 month of Minoxidil, Aminexil in the 3 months before inclusion or ongoing
Systemic treatment affecting hair growth
Any treatment or dietary supplement for antioxidant or capillary

Control group only:

Treatment of general thyroid disorders initiated or modified for less than 3 months prior to inclusion
Treatment of anemia if started less than 3 months before inclusion
General treatment initiated or modified for less than 3 months prior to inclusion in the study