Detection and Pathogenesis of Novel Protein F

Wuhan Asia Heart Hospital

Status: Not yet enrolling

Conditions: Multiple Myeloma; Cardiac Disease

Study type: Observational

Funder types: Other

Identifiers

NCT04338386

YX-2020-B002

Details and patient eligibility

About

From December 6, 2019 to March 23, 2020, the research group of Qingkun Fan found a novel protein(temporarily named protein F) in heparin anticoagulant plasma of three patients with heart disease. One patient was diagnosed with multiple myeloma.However, protein F cannot be detected by serum protein electrophoresis. Preliminary studies have shown that this novel protein F have an obvious absorption peak at about 600nm. Placed at 2-8 degrees for 7 days, protein F will be isolated from heparin plasma. To the naked eye, protein F appear to be transparent jelly between the red blood cells and the plasma. The specific protein F, how it is produced, how it causes disease are still unknown. This study will explore how to detect protein F and how it is produced.

Full description

Protein F screening was performed on anticoagulant blood plasma with heparin Lithium or blood serum by in-house turbidimetric method in Automatic biochemical analyzer.

All patients were followed-up for 3 years by telephones, routine appointment out-patient clinic or returning visit.

Enrollment: 100 estimated patients

Sex: All

Ages: 18 to 100 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Testing total protein and albumin
• Testing routine blood test

Exclusion criteria

• high triglycerides (TG>5.00 mmol/L)
• hyperbilirubinemia (TBIL>170 mmol/L)
• fat emulsion used in past 3 days
• patients with hemolytic disease
• pregnant and lactating women