Detection of Chlamydia DNA by Polymerase Chain Reaction in Primary Tubal and Ovarian Cancers Tissues : a Pilot Study

Ain Shams University

Status: Completed

Conditions: Malignant Neoplasm of Fallopian Tube

Treatments: Other: PCR for chlamydia DNA in primary tubal cancer

Study type: Observational

Funder types: Other

Identifiers

NCT02343510

lab7682

Details and patient eligibility

About

PCR for chlamydia DNA is done in paraffin blocks of cases of primary tubal cancer and is compared to cases of high grade serous ovarian cancer and normal tubes .

Full description

The investigators shall review the early cancer detection unite pathology records for the past 10 years , for cases diagnosed with primary Fallopian tube cancers , cases of high and low grade serous ovarian cancer and cases underwent hysterectomy and salpingectomy for non oncological reasons. Paraffin blocks for suitable selected patients will be examined histologically to confirm the primary diagnosis . Chlamydia DNA in these paraffin blocks of the Fallopian tubes and ovarian tissues will be examined using PCR techniques . The possible correlation between the presence of Chlamydia DNA and the ovarian serous tumours and primary tubal cancer will be analyzed statistically .

Enrollment: 70 patients

Sex: Female

Ages: 40 to 80 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• any patient with primary tubal cancer and high grade serous cancer and normal tubes.

Exclusion criteria

• clinical pelvic inflammatory disease
• salpingectomy for ectopic

Trial design

70 participants in 4 patient groups

primary tubal cancer group

Description:

Paraffin blocks of cases of primary tubal cancer

Treatment:

Other: PCR for chlamydia DNA in primary tubal cancer

high grade serous ovarian cancer group

Description:

Paraffin blocks of cases of high grade serous ovarian cancer

Treatment:

Other: PCR for chlamydia DNA in primary tubal cancer

Low Grade Serous Ovarian Cancer

Description:

Paraffin blocks of cases of low grade serous ovarian cancer

Treatment:

Other: PCR for chlamydia DNA in primary tubal cancer

normal tubes group

Description:

Paraffin blocks of tubes of hysterectomies due to non-oncologic causes

Treatment:

Other: PCR for chlamydia DNA in primary tubal cancer