Detection of G-quadruplex DNA Methylation in Cancer

Zhongnan Hospital

Status

Withdrawn

Conditions

Cell-free DNA

DNA G-quadruplex

Cancer

Methylation

Treatments

Other: G4 DNAm test

Study type

Observational

Funder types

Other

Identifiers

NCT06537063

YX202401

Details and patient eligibility

About

This study aims to evaluate the early diagnosis value of G-quadruplex DNA methylation in patients with cancer.

Full description

Global DNA hypomethylation is a hallmark of most cancers that occurs early during oncogenesis. We would like to investigate whether cancer-specific transcription factor binding to DNA G-quadruplex drives DNA hypomethylation in cancer. In this observational study, we would like to clarify whether DNA hypomethylation around G-quadruplex loci in cell-free DNA could be detected in early-stage cancer patients from healthy donors with high sensitivity and specificity.

Sex

All

Ages

42+ years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

Healthy volunteers identified as asymptomatic during medical examination;
Age≥42 years old;
Voluntarily participate in this study and sign the informed consent.

Exclusion criteria

History of malignancy or having achieved complete remission following tumor surgery;
Certain autoimmune diseases (e.g., systemic lupus erythematosus, Sjögren's syndrome, antiphospholipid syndrome, rheumatoid arthritis, Crohn's disease, IgA nephropathy, myasthenia gravis, dermatomyositis, immune thrombocytopenic purpura, etc.);
Pregnancy;
Acute trauma (including surgical procedures), sudden onset organ failure (e.g., resulting from a car accident, fulminant hepatic failure, acute pancreatitis, severe pneumonia due to influenza virus), or diagnosis of sepsis;
Previous receipt of allogeneic blood transfusion, transplant surgery, or allogeneic cell therapy;
Volunteers who cannot provide informed consent.