Detection Of Monocytes/Macrophages Function And Tim-3 Expression In Septic Patients

Chinese PLA General Hospital (301 Hospital)

Status: Completed

Conditions: SIRS(Systemic Inflammatory Response Syndrome); Sepsis

Study type: Observational

Funder types: Other

Identifiers

NCT01801839

2009BAI86B03 (Other Grant/Funding Number)

Details and patient eligibility

About

The imbalance between anti- and pro-inflammation often occurs in patients with sepsis. And continuous such imbalance could lead to immunoparalysis, which is characterized by loss of delayed type hypersensitivity, failure of primary infections eradication, and a predisposition of secondary nosocomial infections development. However, in which phase of sepsis does immunoparalysis occur is still unknown. So this study aims to tentatively understand this problem by checking monocytes/macrophages' antigen presentation and cytokine secretion，and by checking monocytes/macrophages' expression of Tim-3 (the T cell transmembrane, immunoglobulin, and mucin-3), which is an inhibitory regulatory molecule, in sepsis patients' peripheral blood mononuclear cells.

Enrollment: 1 patient

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• age over 18 years old;
• Fulfilled at least two criteria of systemic inflammatory response syndrome (a) core temperature higher than 38 ℃ or lower than 36 ℃ (b)respiratory rate above 20/min, or carbon dioxide partial pressure below 32 mmHg (c) pulse rate above 90/min, and (d) white blood cell count greater than 12,000/μl or lower than < 4,000/μl or less than 10% of bands.
• Clinically suspected infection;

Exclusion criteria

• were under 18 years of age;
• were refused by the patients or his/her familiality;
• were suffering from acquired immunodeficiency syndrome,viral hepatitis,autoimmune diseases and hematology;
• use medicine which can affect immune system,such as Glucocorticoid and Immunosuppressant
• died within 24h after being taken into the ICU, or refused to get involved in the study, or gave up treatment during the period of observation

Trial design

1 participants in 1 patient group

SIRS,sepsis,normal

Description:

SIRS (1) temperature \> 38 centigrade or \< 36 centigrade; (2) pulse rate \> 90 beats/min; (3) ventilation rate \> 20 breaths/min or hyperventilation with a partial pressure of arterial carbon dioxide (PaCO2) \< 32 mmHg; (4) white blood cell (WBC) count \>1 2,000/μL or \< 4000/μL , or \> 10% immature cells. sepsis SIRS + infection. normal not SIRS and have no infection.