Determination of the Bioavailability of Short Chain Fatty Acids in Healthy Humans

Catholic University (KU) of Leuven

Status: Completed

Conditions: Short Chain Fatty Acids Bioavailability

Treatments: Dietary Supplement: 13C-labeled propionate; Dietary Supplement: 13C-labeled butyrate; Dietary Supplement: Inulin; Dietary Supplement: 13C-labeled Acetate

Study type: Interventional

Funder types: Other

Identifiers

NCT01757379

ML5768

Details and patient eligibility

About

The purpose of the study is to evaluate the bioavailability of acetate, propionate and butyrate in healthy humans using a stable isotope technology.

In addition the level of acetate, propionate and butyrate production from inulin will be determined using the principle of isotope dilution.

Enrollment: 12 patients

Sex: All

Ages: 18 to 65 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Healthy volunteers
• Regular dietary pattern (3 meals/day)
• Age: 18-65y
• BMI: 18,5-27 kg/m2

Exclusion criteria

• Intake of antibiotics 1 month prior to the study
• Abdominal surgery in the past, with the exception of appendectomy
• Intake of medication influencing the gastro-intestinal system 14 days prior to the study
• In treatment at a dietician
• Intake of pre- and/or probiotics
• Exposure to radioactivity 1 year prior to the study
• Serious chronic disease of the gastrointestinal tract
• Use of medication that affects the gastro-intestinal tract during the last 2 weeks prior to the study
• Pregnancy, pregnancy desire or lactation
• Blood donation during the last 3 months prior to the study
• Diabetes (type 1 or 2)
• Aberrant hemoglobin level (Hb) in blood. Normal between 14.0 and 18.0 g/dL for men and between 12.0 and 16.0 g/dL for women

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Single Blind

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12 participants in 4 patient groups

13C-labeled acetate

Experimental group

Treatment:

Dietary Supplement: 13C-labeled Acetate

13C-labeled propionate

Experimental group

Treatment:

Dietary Supplement: 13C-labeled propionate

13C-labeled butyrate

Experimental group

Treatment:

Dietary Supplement: 13C-labeled butyrate

Inulin

Experimental group

Treatment:

Dietary Supplement: Inulin