The Effect of Peak Force At Long and Short Muscle Lengths and the Impact on Muscle Growth and Strength (SLML)

University of British Columbia

Status: Invitation-only

Conditions: Muscle Hypertrophy; Muscular Strength

Treatments: Other: LONG and SHORT Resistance Training; Other: Isokinetic Strength Testing; Other: Maximal Isotonic Strength Testing (1RM); Other: Muscle Oxygenation

Study type: Interventional

Funder types: Other

Identifiers

NCT06666127

H24-01989

Details and patient eligibility

About

The goal of this clinical trial is to learn the effect of applying peak force/resistance to skeletal muscles during resistance exercise training in young and healthy adults. Participants will undergo two types of resistance training conditions that are randomly assigned to the left arm and leg and the right arm and leg. The LONG condition refers to Prime Fitness machines set to setting number '3' which applies peak force at a long muscle length. The SHORT condition refers to Prime Fitness machines set to setting number '2' which applies peak force at a short muscle length.

The main questions the trial aims to answer are:

1. Does applying peak force at a longer or shorter muscle length during resistance exercise training result in greater muscle growth?
2. Does applying peak force at a longer or shorter muscle length during resistance exercise training result in greater muscle growth?

We hypothesize that:

1. LONG will induce greater hypertrophy compared to SHORT conditions
2. LONG will result in greater increases in 1RM strength compared to SHORT
3. LONG and SHORT training will result in angle specific increases in isokinetic strength
4. Muscle oxygenation will decline to a greater degree during LONG compared to SHORT exercise and this will be maintained between weeks 1 and 12 of training

The investigators will compare the change in muscle volume and muscular strength between the LONG and SHORT training conditions, which each participant will have the left arm and leg as well as the right arm and leg randomly assigned to either exclusively LONG or SHORT conditions.

Participants will:

• Resistance train the left and right arm and leg with two separate conditions; LONG and SHORT for 12 weeks, with the first and last weeks dedicated to only strength testing (Isotonic and Isokinetic) and measuring muscle volume via magnetic resonance imaging (MRI)
• Visit the gym for resistance training 2 times per week
• Visit the UBC MRI Research Facility in weeks 1 and 12 for MRI of muscle volume

Full description

Statistical Analysis:

Null Hypothesis Significance Testing (NHST) will be used to measure differences in hypertrophy, differences in 1RM strength, Isokinetic strength and muscle oxygenation between LONG and SHORT conditions.

It is anticipated that this study will require at least 20 participants to be screened and registered into the trial. Data will be analyzed on the basis that all participants will be included in the analysis. Participants who drop out (withdraw from treatment) will be followed up with to collect all available data and will not be replaced. Missing data will be assumed missing at random.

Sample Size Calculation:

Based on the effect size reported by Maeo et al. (2023) for difference in triceps brachia long head hypertrophy between training performed in the lengthened compared to shortened position using a within participant design, (d=0.61) a sample size of 20 participants was calculated to achieve an a priori power of 90%.

Enrollment: 20 estimated patients

Sex: All

Ages: 19 to 30 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

1. Any sex
2. Able to understand and communicate in English
3. 19-30 years of age
4. All "No" answers on the CSEP Get Active questionnaire or doctors' approval to participate
5. Untrained participants: no structured resistance training over the past 12-months (i.e. > 2 hours per week of structured/periodized training)

Exclusion criteria

1. BMI lower than 18 or greater than 30
2. Difficulty to understand and communicate in English
3. Current use of cigarettes or other nicotine devices
4. Any major uncontrolled cardiovascular, muscular, metabolic, and/or neurological disorders
5. Any medical condition impacting the ability to participate in maximal exercise
6. Type one or type two diabetes
7. Diagnosis of cancer or undergoing cancer treatment in the past 12 months
8. Drug therapy with any drugs that alter skeletal muscle metabolism (i.e., Metformin, Benzodiazepines)
9. Omega-3 (EPA, DHA, fish oil) supplementation within the previous 6 weeks
10. Implants containing ferrous metal
11. Surgery or tattoos (including tattooed eyeliner) in the last 6 weeks
12. Pregnancy

Trial design

Primary purpose: Other

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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20 participants in 1 patient group

SHORT and LONG Training Protocol

Experimental group

Description:

Using a within subject design, participants will have the arms and legs from both left and right side of the body (divided by sagittal plane) randomly assigned to either LONG or SHORT conditions. Participants will then engage in maximal strength testing on Prime Fitness machines (1RM) as well as isokinetic strength testing (Biodex) in weeks 1 and 12. Participants will engage in the LONG and SHORT training sessions for 10 weeks in duration from week 2 until week 11.

Treatment:

Other: Muscle Oxygenation

Other: Maximal Isotonic Strength Testing (1RM)

Other: Isokinetic Strength Testing

Other: LONG and SHORT Resistance Training