Dextrose Concentration Decline Rate in Vivo

Dr. Dean Reeves Clinic

Status: Completed

Conditions: Dextrose Concentration

Treatments: Procedure: Intrarticular and subcutaneous dextrose injection with timed measurement of dextrose levels

Study type: Interventional

Funder types: Other

Identifiers

NCT06128122

Reeves-DexConc01

Details and patient eligibility

About

Measuring the speed of decrease in dextrose concentration to help design more accurate in vitro studies

Full description

Rationale: In vitro studies of the potential mechanism of action of therapeutic dextrose injection commonly place human or animal cells in a dextrose (glucose) culture medium for a prolonged period of time, typically 24 hours, and expose them to high dextrose levels, typically 1-25% (1000- 25,000mg%). High glucose levels in culture media can cause cell death. Given that cellular apoptosis/death has not been shown to occur after therapeutic dextrose injection in vivo by histologic analysis, we expect that elevated glucose levels drop rapidly in vivo. In this study 12.5% dextrose will be injected intraarticularly (in the knee) and dextrose 5% will be injected subcutaneously, and dextrose level measurements will be obtained at timed intervals after each in order to produce a curve depicting the pattern and speed of decrease in dextrose levels after in vivo injection. In vitro studies can then utilize this information to alter the pattern and speed of decrease of dextrose levels to mimic in vivo conditions, and in so doing provide more useful data on potential mechanisms of therapeutic dextrose injection in soft tissue and joints.

Enrollment: 1 patient

Sex: Male

Ages: 69 to 69 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

Able to be relatively immobile for intermittent measurement of dextrose levels

Exclusion criteria

Diabetic

Trial design

Primary purpose: Basic Science

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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1 participants in 1 patient group

Dextrose injection intraarticularly and in subcutaneous tissue with timed dextrose measurements

Experimental group

Description:

Step One: Injection of 30 ml of D12.5% in the knee anteromedially with knee flexed. Placement of IV catheter into the suprapatellar pouch, and secure catheter. Withdraw and analyze samples for dextrose levels at 15-minute intervals. If catheter fails, aspirate directly from suprapatellar pouch. Step Two: Inject 1-3 ml of D5W into interstitial space about a functioning Dexcom G7 sensor. Measure dextrose levels every 5 minutes on a Dexcom receiver.

Treatment:

Procedure: Intrarticular and subcutaneous dextrose injection with timed measurement of dextrose levels