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Polyphenols, precisely resveratrol, with red wine as the most substantial source, was associated with improvements in cognitive function. Also, the loss of muscle mass and strength in elderly, that significantly increases dependency of these people, it could be attributed to alterations in gut microbiota through the "gut-muscle axis" and this underline the urgent need to efficiently find out any intervention or preventive approach via modulation of gut microbiota to improve muscle function in elderly. In this context, red wine polyphenols exert their effects through interaction with gut microbiota following the well-known two-way interaction between polyphenols and gut microbiota by promoting the proliferation of beneficial bacteria and increasing their abundance. Similarly, aging cognitive decline can be modulate by microbiota, notably through "gut-brain axis". Additionally, dietary polyphenols can delay inflammation or/and oxidation on the onset of age-related cognitive decline or muscular oxidation or cardiovascular factors risk factors, all of them relevant factors for the onset of physical frailty and dependence in elderly.
Moreover, wine is a singular alcoholic beverage with a high content of phenolic compounds of a very diverse nature on which numerous protective effects on health have been described. In fact, wine, in addition to alcohol, contains a complex mixture of polyphenols, including anthocyanins and non-coloured phenols as proanthocyanidins, flavonols, hydroxycinnamic and hydroxybenzoic acids, stilbenes and lignans. Thus, the bioprotective effects of wine polyphenols could be the consequence of the synergistic effect of this complex mixture of polyphenols from the grape and the winemaking process. That is why it is essential to clarify whether consumption of polyphenols from red wine provided by a nonalcohol red wine within a healthy diet can produce beneficial effects on health, differentiating this pattern from a general consumption of alcohol generally associated with negative effects. Based on the ethical limitations to carry out diet intervention studies with wine in humans, this project proposes the use of a nonalcoholic wine as vehicle of the complex mixture of red wine polyphenols.
The hypothesis of our research is that regular consumption of red wine polyphenols, 150 mg/day, delivered through a nonalcoholic red wine, in the context of a Mediterranean diet (MD pattern), could promote protective mechanisms for a healthy aging, especially through its beneficial effects on cognitive and locomotor abilities and mediated by the modulation of the intestinal microbiota (composition, function and associated metabolome). The main objective of the WinAging project is to add knowledge concerning the diet modulation of molecular mechanisms of the aging process through multi-omic approaches based on the potential health effects of a dietary strategy by a sustained MD supplementation with nonalcoholic red wine rich in polyphenols to tackle cognitive and locomotor abilities in early elderly home-dwelling subjects.
The specific objectives:
Objective 1. To develop a nonalcoholic red wine with high phenolic content and sensorial acceptability.
Objective 2. To evaluate the chronic effects of the intake of wine polyphenols (average dose 150 mg/day) delivered through a nonalcoholic red wine in the context of a MD in early elderly home-dwelling subjects, and applying participatory research to increase adherence of subjects in the clinical intervention study.
Objective 3. To unravel the underlying mechanisms involved in the potential beneficial effects of red wine polyphenols on aging.
Objective 4. To apply integrative computational analyses for the identification of variables (clinical or gut-related) more determinant for a successful prevention of locomotor and cognitive abilities associated with wine polyphenols.
Full description
A total of 72 home-dwelling early elderly volunteers (men and women) 60-74 years old will be included in the intervention (36 in each arm of the intervention).
During the study there will be 11 visits in total. Visits will be every 4 weeks form the V1. The study visits will be the following:
Screening visit (V0).
Basal visit (V1).
Visits during intervention (V2, V3, V4, V5, V6, V7, V8, V9).
3-month study visit (V4)
6-month study visit (V7)
Telephone visits (V2, V3, V5, V6, V8, V9)
Final study visit (V10). In visits V0, V1, V4, V7 and V10 volunteers must present themselves in fasting conditions of 8 hours to obtain fasting blood samples.
Basal visit (V1; week 0):
Nuclear Magnetic Resonance (NMR) and muscle biopsy (±5 days V1)*.
Isokinetic assessment (±5 days V1)*
Revision of study clinical history.
Vital signs (blood pressure/resting heart rate).
Checking the concomitant medication.
Anthropometry waist circumference; hip; body weight and composition; height).
Checking the physical activity and sedentary habits (IPAQ-E).
Checking the quality of life (SF-36 Health survey questionnaire)
Checking depression symptoms (Geriatric Depression Scale Questionnaire)
Checking MD adherence
Checking the cognitive and locomotor abilities
Spanish validated of the Mini Mental State Examination (MMSE), Alzheimer disease questionnaire (AD8), Memorey Alteration tets (TAM test), Fototest
Sarcopenic parameters (muscle strength based on grip dynamometry; skeletal muscle mass index (SMI) based on bioimpedance analysis (BIA), kg/m2 and appendicular skeletal muscle mass index (ASMI) based on bioimpedance analysis (BIA), low physical performance or physical function based on 4 m gait speed, m/s.
Blood sample extraction.
Ultrasound (muscle mass and abdominal fat).
Checking the 3-day dietary record and Food Frequency Questionnaire (FFQ).
Collection of feaces samples.
Collection of saliva samples.
Collection of urine samples.
MD guidelines explanation.
Schedule the next visit and instructions.
Administration of red nonalcoholic wine through opaque bottles to protect from light.
In a subsample of n= 15 subjects/group, 5 days before or after the visit V1, volunteers must have a:
Telephone visits: (V2 and V3; week 4 and 8):
Checking the FFQ
Checking changes in concomitant medication
Product intake control
Record adverse effects
Schedule the next visit and instructions.
3-month study visit (V4; week 12):
Telephone visits: (V5 and V6; week 16 and 20):
Checking the FFQ
Checking changes in concomitant medication
Product intake control
Record adverse effects
Schedule the next visit and instructions. 6-month study visit (V7; week 24):
Telephone visits: (V8 and V9; week 28, and 32):
Final visit (V10; week 36):
NMR and muscle biopsy (±5 days V1)*.
Isokinetic assessment (±5 days V1)*
Revision of study clinical history.
Vital signs (blood pressure/resting heart rate).
Checking the concomitant medication.
Anthropometry waist circumference; hip; body weight and composition; height).
Checking the physical activity and sedentary habits (IPAQ-E).
Checking the quality of life (SF-36 Health survey questionnaire)
Checking depression symptoms (Geriatric Depression Scale Questionnaire)
Checking MD adherence
Checking the cognitive and locomotor abilities
Spanish validated of the MMSE, AD8, TAM test, Fototest
Sarcopenic parameters (muscle strength based on grip dynamometry; skeletal muscle mass index (SMI) based on bioimpedance analysis (BIA), kg/m2 and appendicular skeletal muscle mass index (ASMI) based on bioimpedance analysis (BIA), kg/m2; low physical performance or physical function based on 4 m gait speed, m/s.
Blood sample extraction.
Ultrasound (quadricep muscle mass and abdominal fat).
Checking the 3-day dietary record and Food Frequency Questionnaire (FFQ).
Collection of feaces samples.
Collection of saliva samples.
Collection of urine samples. *In a subsample of n= 15 subjects/group, 5 days before or after the visit V10.
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72 participants in 2 patient groups, including a placebo group
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Central trial contact
Anna Pedret Figuerola, Dr.; Rosa Solà Alberich, Professor
Data sourced from clinicaltrials.gov
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