Differential Diagnostics of Etiology of Acute Infections

The Republican Research and Practical Center for Epidemiology and Microbiology

Status

Completed

Conditions

Neuroinfections

Intestinal Infectious Diseases

Study type

Observational

Funder types

Other

Identifiers

NCT03161951

RRPCEM_NMAT

Details and patient eligibility

About

Method for diagnostics of the origin of infections (bacterial vs viral) based on the identification of activation markers of blood neutrophils and monocytes will be developed.

Full description

During the study, method for diagnostics of the origin of infections (bacterial vs viral) based on the identification of activation markers of blood neutrophils and monocytes will be developed.This method will allow to make a diagnosis within an hour after blood sampling.

The expression of the following markers of activation will be studied using flow cytometry: CD11b, CD11c, CD13, CD16, CD32, CD35, CD38, CD62L, CD64, CD66b, CD88, CD284, HLA-ABC and HLA-DR.

Enrollment

169 patients

Sex

All

Ages

18 to 80 years old

Volunteers

No Healthy Volunteers

Inclusion criteria

neuroinfections or acute intestinal infections;
the possibility of blood sampling within a period of up to 24 hours from the moment of admission to hospital;
less than 72 hours passed after the onset of the disease and until blood was collected;

Exclusion criteria

refuse of patient to participate in the trial;
more than 24 hours passed after the patient entered the hospital;
the disease began more than 72 hours ago;
concomitant severe acute and chronic diseases;
malignant neoplasms, treatment at a time of less than 1 year, autoimmune diseases in the phase of exacerbation;
pregnancy/lactation;
HIV, Hepatites B/C;
active tuberculosis;
alcohol use disorder/drug addiction.