Digital PCR for Quantifying Periodontal Pathobionts (dPCR)

University of Ljubljana

Status: Completed

Conditions: Periodontal Diseases

Treatments: Diagnostic Test: digital PCR

Study type: Observational

Funder types: Other

Identifiers

NCT06692595

J7-4639 (Other Grant/Funding Number)

UKCdPCR1

Details and patient eligibility

About

Comparison of quantitative polymerase chain reaction (qPCR) and digital polimerase chain reaction (dPCR) quantification of subgingival plaque samples.

Enrollment: 40 patients

Sex: All

Ages: 20 to 80 years old

Volunteers: Accepts Healthy Volunteers

Inclusion and exclusion criteria

For inclusion, each subject had to be systemically healthy and aged 20 to 80 years. Subjects were excluded if they: had less than 5 teeth in each jaw quadrant, regularly used a mouthwash as part of their oral hygiene routine, had received antibiotics during the last 6 months or during the study period, were pregnant or lactating, had a known systemic illness (i.e. HIV/AIDS, diabetes mellitus, cancer, diseases of bone metabolism, diseases affecting wound healing) or were treated with immunosuppressants, chemotherapy, radiation, calcium antagonists, antiepileptics and non-steroidal anti-inflammatory drugs.

Trial design

40 participants in 2 patient groups

Periodontitis patients

Treatment:

Diagnostic Test: digital PCR

Individuals with healthy periodontium

Treatment:

Diagnostic Test: digital PCR