DNA Analysis of Tissue Samples From Patients With Head and Neck Cancer

Vanderbilt University Medical Center

Status: Completed

Conditions: Head and Neck Cancer

Treatments: Other: laboratory biomarker analysis; Genetic: cytogenetic analysis; Other: immunohistochemistry staining method; Genetic: mutation analysis; Genetic: polymerase chain reaction; Genetic: gene expression analysis

Study type: Observational

Funder types: Other; NIH

Identifiers

NCT00898664

VU-VICC-IRB-060462

VU-VICC-HN-0625

VICC HN 0625

Details and patient eligibility

About

RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.

PURPOSE: This laboratory study is looking at DNA in tissue samples from patients with head and neck cancer.

Full description

OBJECTIVES:

• Assess common genetic mutations in tumor and germline DNA samples from patients with head and neck squamous cell carcinoma (HNSCC).
• Search for viral DNAs and relate this information to demographics, tumor characteristics, and patient prognosis.
• Search for novel viral sequences and cancer-associated gene mutations in young nonsmoking HNSCC patients and in other distinct head and neck cancers.
• Determine tumor suppressor gene mutations in HNSCC patients and relate this information to gene expression data.
• Validate gene expression information using independent techniques such as real-time PCR on expressed sequences and immunohistochemistry.

OUTLINE: Genetic mutation analysis and gene expression studies are performed on tissue samples from patients with head and neck squamous cell carcinoma. Techniques used include real time-PCR and immunohistochemistry.

Enrollment: 74 patients

Sex: All

Ages: 18+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Patient with head and neck squamous cell carcinoma (HNSCC) enrolled in the Head and Neck Tissue Repository

  • Sufficient biological material collected to perform study tests

• Historical material available from excess tissues at the Vanderbilt Pathology Department

Exclusion criteria

• Not specified

PRIOR CONCURRENT THERAPY:

• Not specified