DNA Analysis of Tumor Tissue From Patients With Acute Myeloid Leukemia

ECOG-ACRIN Cancer Research Group

Status: Completed

Conditions: Leukemia

Treatments: Other: immunologic technique; Genetic: microarray analysis; Genetic: fluorescence in situ hybridization; Genetic: comparative genomic hybridization; Genetic: polymerase chain reaction; Other: diagnostic laboratory biomarker analysis; Genetic: DNA methylation analysis

Study type: Observational

Funder types: NETWORK; NIH

Identifiers

NCT00897936

CDR0000476571

ECOG-E1900T2

Details and patient eligibility

About

RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. It may also help doctors predict how well patients respond to treatment.

PURPOSE: This laboratory study is looking at tissue samples from patients with acute myeloid leukemia.

Full description

OBJECTIVES:

Primary

• Determine gene expression, genome integrity, cytosine methylation, and chromatin structure in patients with normal cytogenetics leukemia (NCL) acute myeloid leukemia.
• Determine whether NCL can be deconstructed into specific disease entities by analysis of the integrated genomic and epigenomic datasets using supervised and unsupervised methods in these patients.
• Identify the gene pathways that define NCL subtypes and molecular targets for validation in preclinical and clinical trials for these patients.
• Determine whether integrated analysis provides markers of prognostic and therapeutic response that accurately predicts clinical outcome and can be used to select patients for risk-stratified therapeutic trials.

OUTLINE: This is a pilot, multicenter study.

Samples are analyzed to assess array comparative genomic hybridization using polymerase chain reaction (PCR) and fluorescent in situ hybridization; chromatin immunoprecipitations (chip) using PCR; Hpa II tiny fragment enrichment by ligation-mediated PCR (HELP) using DNA methylation analysis; and gene expression profiling.

PROJECTED ACCRUAL: A total of 32 patients will be accrued for this study.

Enrollment: 418 patients

Sex: All

Ages: 18 to 120 years old

Volunteers: No Healthy Volunteers

Inclusion and exclusion criteria

DISEASE CHARACTERISTICS:

• Diagnosis of acute myeloid leukemia
• Enrolled on clinical trial ECOG-E1900

PATIENT CHARACTERISTICS:

• Not specified

PRIOR CONCURRENT THERAPY:

• Not specified