DNA Methylation Profile of the SOCS-1 Gene Promoter in Smokers Patients With Chronic Periodontitis.

University of Sao Paulo

Status: Completed

Conditions: Periodontal Diseases; Epigenetics

Treatments: Genetic: oral epithelial cells obtained by rinsing with 3% sucrose

Study type: Observational

Funder types: Other

Identifiers

NCT03371446

CAAE: 57171816.2.0000.5419

Details and patient eligibility

About

Periodontitis is related to host genetics, constitution of the dental biofilm and environmental factors such as smoking. DNA methylation is a mechanism of genetic expression that can inhibit or silence gene expression. In this way several researchers have been dedicated to study the genetic influence on the susceptibility and / or increased risk to periodontal disease. Studies have reported association between several epigenetic biomarkers with periodontal inflammation. Considering the hypothesis that there is an association between smoking and methylation in genes related to periodontal disease, the objective of this study was to verify the DNA methylation pattern in oral epithelial cells of patients with chronic periodontitis (CP) in the promoter of a specific gene involved in the control of inflammation, as suppressor of cytokine signaling (SOCS) 1 in smokers and nonsmokers patients.

Full description

This was an experimental type study with parallel controls, comparing two groups, a group with consumption of 10 minimum cigarettes per day, with a diagnosis of chronic periodontitis. And another control group were non-smokers with chronic periodontitis. For this, genomic DNA was purified from oral epithelial cells obtained by rinsing with 3% sucrose, for a single time of collection, patients received periodontal treatment after collection and data analysis. The DNA was modified by Sodium bisulfite and the methylation patterns of the DNA were analyzed with the MSPCR technique (Polymerase chain reaction). This study was approved by the Institutional Review Board of the School of Dentistry of Ribeirão Preto (CAAE:57171816.2.0000.5419 ), and all patients need to provide written informed consent.

Enrollment: 60 patients

Sex: All

Ages: 30+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Chronic Periodontitis carriers - presence of proximal insertion loss ≥5 mm in more than 30% of the teeth present.
• Periodontal pocket ≥ 5mm
• Smokers who have smoked 10 or more cigarettes per day for the past five years.
• No positive history of basic periodontal treatment in the last six months.

Exclusion criteria

• Extensive prosthetic involvement.
• Using anti-inflammatories.
• Pregnant or nursing.
• Presence of systemic alterations that compromise host response or require prophylactic medication for treatment.
• History of constant use of oral antiseptics in the last six months.

Trial design

60 participants in 1 patient group

Smokers/Non-smokers

Description:

It was an experimental study with parallel controls, comparing two groups, a group with patients who smoked for more than 10 years, consuming 10 more cigarettes per day and diagnosing chronic periodontitis (case) and another group (control) were non-smokers with chronic periodontitis, according to the standard of World Health Organization (WHO) definition of the smoking population

Treatment:

Genetic: oral epithelial cells obtained by rinsing with 3% sucrose