Does Biological Sex Influence Insulin Sensitivity and Muscle Metabolism Following High-intensity Interval Exercise?

University of Toronto

Status: Completed

Conditions: Exercise

Treatments: Other: Seated Rest (Non-exercise control); Other: High-intensity interval exercise

Study type: Interventional

Funder types: Other

Identifiers

NCT06074003

44435

Details and patient eligibility

About

High-intensity interval exercise (HIIE) is a type of exercise that involves alternating periods of intense exercise with periods of rest. HIIE has been shown to improve many aspects of cardiovascular and metabolic health in a time-efficient manner (e.g., only 20 minutes per exercise session). An important health benefit of exercise is improved blood sugar control, which can help reduce the risk of metabolic diseases like type 2 diabetes. A single session of HIIE has been shown to improve blood sugar in males, but it is unknown if females achieve the same health benefit. It is also not fully understood how exercise improves blood sugar in males and females. Therefore, the purpose of this project is 1) to determine if a single session of HIIE improves blood sugar control in males and females, and 2) to evaluate if changes in skeletal muscle can explain the beneficial effects of HIIE on blood sugar.

Full description

The primary purpose of this study is to determine if biological sex influences the effects of high-intensity interval exercise (HIIE) on insulin sensitivity and muscle mechanisms. The investigators will measure insulin sensitivity and muscle outcomes of participants on two separate occasions: 1) Following 30 minutes of sitting in the lab; and 2) Following a single session of HIIE on a stationary bike. Insulin sensitivity will be measured by taking blood samples after participants consume a sugary drink. Muscle outcomes will be measured by taking a small amount of muscle from the vastus lateralis (thigh) muscle. Groups of male and female participants will be recruited and tested using best practice guidelines for sex-based comparisons of exercise responses.

The study will advance knowledge regarding the potential for biological sex to influence the metabolic responses high-intensity exercise.

Enrollment: 24 patients

Sex: All

Ages: 18 to 35 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• 18-35 yrs
• VO2peak considered recreationally active as defined as 'fair' or 'good' (males: 41.6- 50.5ml/kg/min; females: 35-41.9 ml/kg/min) based on Canadian Society for Exercise Physiology (CSEP) normative fitness values.
• BMI between 18-27 kg/m2
• 1-3 structured exercise sessions/week, and not training for any specific sport
• Weight stable (within ± 2kg for at least 6 months)
• Eumenorrheic (female only), defined as menstrual cycle lengths ≥ 21 days and ≤ 35 days resulting in 9 or more consecutive periods per year)
• Non-smoker

Exclusion criteria

• Diagnosed with cardiovascular or metabolic disease, hyper- or hypogonadism, and/or polycystic ovarian syndrome (PCOS)
• The use of medication for managing blood glucose or lipid metabolism
• Current use of oral contraceptives or use within the last 3 months
• Irregular menstrual cycles (<21 days or >35 days)
• Pregnant, lactating, or menopausal
• Recreational smoking of any kind
• Inability to perform the study exercise protocols or follow the pre-trial dietary or physical activity controls
• Taking medications affecting substrate metabolism (corticosteroids or nSAIDs)
• Actively engaging in a low-carbohydrate diet (e.g., ketogenic, Atkins)

Trial design

Primary purpose: Basic Science

Allocation: Non-Randomized

Interventional model: Crossover Assignment

Masking: None (Open label)

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24 participants in 2 patient groups

Females

Experimental group

Description:

Female participant group

Treatment:

Other: High-intensity interval exercise

Other: Seated Rest (Non-exercise control)

Males

Experimental group

Description:

Male participant group

Treatment:

Other: High-intensity interval exercise

Other: Seated Rest (Non-exercise control)