Dose and Response of Dietary Nitrate in Healthy and Active Men

Swiss Paraplegic Research, Nottwil

Status: Completed

Conditions: Healthy

Treatments: Dietary Supplement: Beetroot juice; Dietary Supplement: sodium nitrate

Study type: Interventional

Funder types: Other; NETWORK

Identifiers

NCT01961427

2013-12

Details and patient eligibility

About

It was shown by some researchers that dietary nitrate such as beet root juice can lower oxygen consumption in men and act as a performance enhancing supplement.

It was not shown, what the optimal dosage is and if there is any difference in oxygen consumption comparing beet root juice and inorganic nitrate.

Therefore, the aim of the study is to conduct a study ingesting different sodium nitrate and beet root juice dosages. We would measure nitrate and nitrite concentrations in the blood at different time points and the subjects have to perform an exercise test measuring oxygen consumption 3 hours after the ingestion of nitrate.

Full description

1. The subjects have to ingest 7 different dosages of either beetroot juice or sodium nitrate on seven different occasions. Every hour a blood withdrawal is taken to measure nitrate and nitrite concentration in the blood.
2. The subjects have to ingest 7 different dosages of either beetroot juice or sodium nitrate on seven different occasions. Three hours after the intake of the supplement the subjects have to perform an exercise test on a bicycle. They have to cycle 5 min at 50% of maximal oxygen consumption and 8min at 80% of maximal oxygen consumption separated by a 5min break. Oxygen consumption would be measured during the whole exercise test. Blood withdrawal is taken before the ingestion, after 3 hours and after the test.

Enrollment: 21 patients

Sex: Male

Ages: 18 to 45 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• age 18 to 45
• healthy active men
• non-smoking

Exclusion criteria

• medication intake during study phase

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Factorial Assignment

Masking: Triple Blind

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21 participants in 7 patient groups

sodium Nitrate 12mmol

Active Comparator group

Description:

Ingestion of a solution of inorganic nitrate, dosage: 12mmol

Treatment:

Dietary Supplement: sodium nitrate

sodium Nitrate (6mmol)

Active Comparator group

Description:

ingestion of inorganic nitrate (6mmol solution)

Treatment:

Dietary Supplement: sodium nitrate

sodium nitrate 3mmol

Active Comparator group

Description:

ingestion of inorganic nitrate (3mmol solution)

Treatment:

Dietary Supplement: sodium nitrate

sodium nitrate (0mmol)

Active Comparator group

Description:

Ingestion of water as a placebo

Treatment:

Dietary Supplement: sodium nitrate

Beetroot juice (12mmol)

Active Comparator group

Description:

ingestion of 170ml of concentrated beetroot juice (12mmol)

Treatment:

Dietary Supplement: Beetroot juice

beetroot juice (6mmol)

Active Comparator group

Description:

ingestion of 85ml concentrated beetroot juice (6mmol)

Treatment:

Dietary Supplement: Beetroot juice

Beetroot juice (3mmol)

Active Comparator group

Description:

Ingestion of 42.5ml of concentrated beetroot juice (3mmol)

Treatment:

Dietary Supplement: Beetroot juice