Drug Sensitivity Screening for Gastrointestinal Cancer

Peking University Cancer Hospital & Institute

Status: Unknown

Conditions: Gastrointestinal Cancer Metastatic

Treatments: Diagnostic Test: Personalized drug sensitivity test

Study type: Interventional

Funder types: Other

Identifiers

NCT04298489

PKUCH-M01

Details and patient eligibility

About

Explore the clinical feasibility of using primary cell culture system to guide gastrointestinal cancer chemotherapy, and establish the correlation between ex vivo drug sensitivity and patient clinical response.

Study objectives: Personalized drug sensitivity test for late stage,potentially operable gastrointestinal cancer using patient derived primary cell culture.

Explore the clinical feasibility of using primary cell culture system to guide gastrointestinal cancer chemotherapy, and establish the correlation between ex vivo drug sensitivity and patient clinical response.

The study will collect primary tumor tissues from stage III/IV gastrointestinal cancer patients who underwent emergency surgeries, and then establish the primary tumor cell library for ex vivo chemotherapy drug sensitivity test in order to:

1. Compare the ex vivo Maximal Inhibition Index(MI) and Drug Sensitivity Index (DSI) with patient's Overall Response Rate (ORR)
2. Provide research support for future clinical treatment.

This ex vivo method applies to single or combination drug regimen, and does not require prior knowledge of the specific mechanism for individual patient's drug sensitivity. Previous research as well as literature studies support the close relationship between ex vivo drug sensitivity and in vivo drug response.

Full description

The patient underwent surgery to remove tumor and agreed to take out the abdominal tumor specimens for research. A section of each sample was removed for the generation of PDX models as described early.The rest of the tumor cells were expanded using ex vivo drug sensitivity assay.

The drugs used in the study were 5-fluorouracil, Oxaliplatin, and irinotecan at C0 = 10 mM, 2.5 mM and 0.02 mM, respectively. They were applied either as single agents or in combinations of 5-fluorouracil and Oxaliplatin, 5-fluorouracil and irinotecan, or all three agents, or others. After 7 days of treatment, the tumor cells were stained with EdU, Hoechst and EpCAM with the Cell Quantitative Detection Kit. Images were acquired with an automated microscopic image-scanning system and analyzed with the built-in software.

Enrollment: 40 estimated patients

Sex: All

Ages: 18 to 70 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

1. Adult patients between 18 and 70 years old, male or female;
2. Voluntary patient consent；
3. Treatment-naïve, stage III/IV gastrointestinal cancer patients with pathology confirmation；
4. According clinical researcher, patient has operable tumor lesion, and tumor tissue can be obtained through surgical removal;
5. Good tolerability to standard chemotherapy regimen；
6. ECOG status <3；
7. Estimated survival time no less than 6 months；
8. Patient has at least one measurable disease lesion (according to RECIST1.1).

Exclusion criteria

1. Patient has received any prior anti-cancer treatment;
2. Participated in any other clinical study within 6 months;
3. Women currently breast feeding or pregnant;
4. Severe liver or kidney function impairment (Live function: TBIL ≤1.5×ULN，ALT & AST≤2.5×ULN);
5. Patients with liver metastasis ≤5.0×ULN；Kidney function：Cr ≤1.5×ULN and creatinine clearance rate≥ 50 mL/min (according to the Cockcroft-Gault formula);
6. Patients with cognitive impairment, psychological disease, or poor compliance;
7. Allergic to known chemotherapy ingredients;
8. Other factors researchers deemed not suitable for study participation.

Trial design

Primary purpose: Diagnostic

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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40 participants in 1 patient group

stage III/IV gastrointestinal cancer patients

Experimental group

Description:

The study group (Personalized drug sensitivity test) was treated according to the physician's opinion. Tumor tissues are obtained during the surgery or via biopsy with informed consent, for the purpose of ex vivo assay.

Treatment:

Diagnostic Test: Personalized drug sensitivity test