E. Faecalis Prevalence in Primary and Secondary Endodontic Infections: a Pre-post Microbial Analysis Following Chemo-mechanical Preparation

University of Siena

Status: Completed

Conditions: Apical Periodontitis

Treatments: Procedure: Root Canal Treatment

Study type: Observational

Funder types: Other

Identifiers

NCT04637659

EF001

Details and patient eligibility

About

Post-treatment apical periodontitis is a fairly prevalent condition frequently caused by a persistent endodontic infection due to failure of the endodontic treatment. Microbial species in treated or untreated root canals were found to be different, with the former being less diverse and mainly characterized by Gram positive, facultative anaerobes bacteria than the latter. Enterococcus faecalis is the most frequently detected species in root-filled teeth in many studies (Zhang et al., 2015). In fact, E. Faecalis retains many virulence factors allowing adhesion to host cells and extracellular matrix, tissue invasion and damage through toxins release, as well as the ability to survive even in harsh environmental conditions. Further studies are needed to clarify E. faecalis prevalence among the different forms of pulpal and periapical lesions as well as its correlation with clinical symptoms.

Enrollment: 72 patients

Sex: All

Ages: 18 to 70 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• between the ages of 18 and 70;
• good systemic health.
• patients in need of an endodontic treatment/retreatment

Exclusion criteria

• taking antibiotic therapy in the previous 3 months;
• presence of communication between the canal system and the oral cavity;
• teeth that are not perfectly isolated;
• teeth with probing depth >4 mm with positive BOP.

Trial design

72 participants in 5 patient groups

Healthy non-treated teeth

Description:

Healthy teeth being treated for restorative or prosthodontics reasons

Treatment:

Procedure: Root Canal Treatment

Healthy treated teeth

Description:

Teeth being retreated for restorative or prosthodontics reasons

Treatment:

Procedure: Root Canal Treatment

Irreversible pulpitis

Description:

Teeth being treated because of a poor pulpal status

Treatment:

Procedure: Root Canal Treatment

Post-treatment apical periodontitis

Description:

Teeth being retreated due to the presence of an apical lesion

Treatment:

Procedure: Root Canal Treatment

Necrosis

Description:

Teeth being treated because of pulpal necrosis

Treatment:

Procedure: Root Canal Treatment