Early Embryo Cleavage And Multiple Pregnancies After ICSI

Al-Azhar University

Status and phase

Completed

Phase 2

Phase 1

Conditions

Infertility

Treatments

Procedure: transfer non early cleavage embryo

Procedure: transfer early cleavage embryos

Study type

Interventional

Funder types

Other

Identifiers

NCT02265055

u12345

Details and patient eligibility

About

To observe whether Number of early cleavage embryos can be a predictor for multiple pregnancies after ICSI.

Full description

A total of 193 infertile couples, attending the ART unit, International Islamic Center for Population Studies and Research (IICPSR), -Al- Azhar University in the period from December 2010 to September 2012, were included in this study.

The controlled ovarian hyperstimulation protocol was performed according to a long GnRH agonist protocol starting in the midluteal phase (day 21) of the preceding cycle. Embryos were assessed at 25 - 27 hr after ICSI for early cleavage. Embryos which reached the two cell stage at this interval were classified as Early Cleavage (EC) embryos, and the remaining as Non Early Cleavage (NEC) embryos. Embryos were assessed again at 64 - 68 hours post-ICSI for day-three embryo morphology. Day 3 embryo transfer was done. The best two or three embryos, according to day 3 embryo morphology were transferred. The patients were subdivided into two subgroups; one will transfer early cleavage embryos (EC) and the other will transfer non early cleavage embryo (NEC).

Enrollment

193 patients

Sex

Female

Ages

Under 35 years old

Volunteers

No Healthy Volunteers

Inclusion criteria

BMI >25 - 30 kg/m2
Have two ovaries
Regular cycles
first ICSI cycle
Long midluteal GnRH protocol.

Exclusion criteria

uterine factor as a cause of female factor infertility
pelvic masses or diseases (e.g: endometriosis, fibroids, hydrosalpnix, ...)
history of medical disorders (e.g: hypertension, D.M, thyroid dysfunction, liver diseases, renal diseases,...)
azoospermia as a cause of male factor infertility