Blood DdPCR for Early Identification and Dynamic Surveillance of Pathogenic Bacteria in ICU Septic Patients: a Single-centre, Prospective, Observational Study

Huazhong University of Science and Technology

Status: Enrolling

Conditions: Bloodstream Infections; Critically Ill Intensive Care Unit Patients; Sepsis

Study type: Observational

Funder types: Other

Identifiers

NCT06791889

WHUICU202409

Details and patient eligibility

About

The purpose of this study is to investigate the efficacy of droplet digital PCR (ddPCR) in identifying pathogenic organisms in ICU sepsis patients, aiming to find a method that allows early identification of pathogenic organisms and dynamic surveillance in order to assess the association between pathogenic species and loads and clinical characteristics and outcomes.

Full description

This is a monocentric observational study. Patients admitted to the ICU with a first diagnosis of sepsis will be dynamically monitored for nucleic acid loads of pathogenic bacteria in the blood using ddPCR at different time points until the last blood sample is taken on day 28-30 or the patient is discharged from the ICU.

Enrollment: 60 estimated patients

Sex: All

Ages: 18+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Patients with first diagnosis of sepsis after ICU admission

Exclusion criteria

• Age under 18 years
• Do not meet Sepsis 3.0 diagnostic criteria
• Refusal to sign informed consent

Trial design

60 participants in 1 patient group

ddPCR group