Eccentric Cycling Exercise on Mitochondrial Function of Lymphocyte

Chang Gung Medical Foundation

Status: Completed

Conditions: Eccentric Exercise Training

Treatments: Behavioral: Concentric cycling training; Behavioral: Eccentric cycling training

Study type: Interventional

Funder types: Other

Identifiers

NCT06576804

202100004A3

Details and patient eligibility

About

Eccentric cycling exercise (ECE) features lower metabolic demand and higher improvement of muscle strength compared to traditional concentric cycling exercise (CCE). Mitochondria can regulate energy metabolism and adaptive immune quality in T lymphocytes. However, the effects of ECE on mitochondrial functions in T-lymphocytes have not yet been established.

Method: A total of 33 healthy sedentary males were randomized and divided into ECE (n=11), CCE (n=11), and control groups (n=11). These subjects progressively performed CCE or ECE from 60% to 80% maximal workload on a bicycle ergometer for 40 min/day, 5 days/week for 6 weeks. A graded exercise testing and an isokinetic strength test were conducted to evaluate cardiopulmonary fitness and muscle strength, respectively. Phenotypes and mitochondrial respiratory capacity in T lymphocyte were analyzed using flow cytometry and high-resolution respirometer, respectively.

Full description

Eccentric exercise training (EET) increases physical performance while having lower metabolic demand than concentric exercise training (CET).

Mitochondria of lymphocytes are essential for cell proliferation, death, and differentiation, and play a critical role in establishing lymphocyte phenotypes and their functions.

Whether EET influences lymphocyte bioenergetic efficiency remains unclear. The study was to investigate the effects of ECE and CCE regimens on adaptive immune functions and mitochondrial bioenergetics of T lymphocytes in sedentary males.

Enrollment: 33 patients

Sex: Male

Ages: 20 to 30 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

1. Sedentary lifestyle.
2. Nonsmokers, nonusers of medications/vitamins.
3. No any cardiopulmonary/hematological risks.

Exclusion criteria

1. Have regular exercise habits (i.e., exercise frequency once per week, duration >20 min).
2. Have retabolic or musculoskeletal disease.

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Single Blind

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33 participants in 3 patient groups

Eccentric cycling training

Experimental group

Description:

Moderate to high intensity cycling training in eccentric type

Treatment:

Behavioral: Eccentric cycling training

Concentric cycling training

Experimental group

Description:

Moderate to high intensity cycling training in concentric type (intensity matched the eccentric training)

Treatment:

Behavioral: Concentric cycling training

Control group

No Intervention group

Description:

without receiving any exercise training