Effect of Abstinence Duration on Embryo Development

Universitair Ziekenhuis Brussel

Status: Completed

Conditions: Embryo Development

Treatments: Other: second masturbation

Study type: Interventional

Funder types: Other

Identifiers

NCT03323892

2017/271

Details and patient eligibility

About

The aim the investigators study is to analyze the embryo development if oocytes of the participants are injected with sperm from a longer or shorter (2 hours) abstinence duration.

Full description

Participants undergoing an IVF treatment, are asked to respect an abstinence duration of 2-7 days. This is to obtain a sufficient number of motile sperm. A recent investigation, analyzed the quality (number and motility) of a sperm sample produced after an abstinence duration of 4 to 7 days, followed by a second sperm sample 2 hours later. The second sample (after 2 hours) was able to produce sperm cells with a higher motility. Not only has this short abstinence duration of 2 hours a positive effect on the motility, it will also reduce the sperm DNA damage, which again has a positive effect on embryo development. Because participants need gametes of high quality to obtain embryos with a high quality, the investigators want to investigate what the effect is of abstinence duration on embryo development.

Enrollment: 100 patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Patients planned for embryo development up to day 5
• at least 6 mature oocytes
• fresh ejaculates
• ICSI
• fresh embryo transfer or freeze all strategy
• all stimulation protocols
• concentration of >1x10^6 /ml sperm

Exclusion criteria

• IVF
• IVF versus ICSI patients
• chirurgical sperm
• acceptors of donor oocytes
• patients with PGD
• patients already enrolled in another study

Trial design

Primary purpose: Diagnostic

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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100 participants in 2 patient groups

'normal' abstinence duration

Active Comparator group

Description:

Patients follow the normal abstinence duration as asked by the physician (2-7 days). This sperm will be used to inject half of the oocytes Intervention = second sperm sample

Treatment:

Other: second masturbation

short abstinence duration

Experimental group

Description:

Patients will be asked to produce a second sperm sample, 2 hours after the first. This sperm will be used to inject the other half of the oocytes. Intervention = second sperm sample

Treatment:

Other: second masturbation