Effect of Acute Bout of Exercise on Levels of PAHSA (ETAPA)

Lenka Rossmeislova

Status: Completed

Conditions: Insulin Sensitivity

Treatments: Other: Fasting control; Other: Exercise

Study type: Interventional

Funder types: Other

Identifiers

NCT05572905

NU21-01-00469

Details and patient eligibility

About

Exercise represents an important tool in the prevention and treatment of metabolic disorders associated with obesity and aging, such as type 2 diabetes and cardiovascular disease. Besides skeletal muscle and its myokinins, the metabolic effects of exercise also rely on the induction of favorable changes in adipose tissue function. For example, adipose tissue is a source of lipokinins from the family of palmitic acid esters of hydroxy fatty acids (PAHSA), which have anti-inflammatory and insulin-sensitizing properties. We have recently shown that 4 months of exercise training increases PAHSA levels in adipose tissue and circulation. However, the mechanisms involved in the induction of PAHSA levels in response to exercise are unknown. The aim of the Effect of Acute Bout of Exercise on Levels of PAHSA (ETAPA) project is therefore to investigate the regulation of PAHSA metabolism in response to both acute and chronic exercise. To achieve this goal, we will employ state-of-the-art analytical methods to measure PAHSA levels in both adipose tissue and circulation of subjects of various ages and adiposity status. The main output of the ETAPA project will be the proof of principle regarding the important role of PAHSA lipokinins in exercise-induced enhancement of insulin sensitivity and the identification of potential drug targets that could be used to further improve PAHSA metabolism for the treatment of metabolic disorders associated with aging or obesity.

Enrollment: 60 patients

Sex: All

Ages: 25 to 80 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• healthy young lean, young obese and older omnivorous men and women as defined by BMI, self-reported activity, self-reported medical history and self-reported diet assessment
• must be able to withstand repeated blood draws
• must be able to undergo abdominal fat biopsy

Exclusion criteria

• use of betablockers
• use of glucocorticoids
• use of non-steroidal anti-inflammatory drugs
• use of metformin in prediabetes
• use of psychiatric drugs such as selective serotonin reuptake inhibitors, serotonin and norepinephrine reuptake inhibitors, anticonvulsants and others
• oncologic malignancy
• chronic inflammatory or autoimmune diseases
• diabetes mellitus
• chronic ischemic heart disease
• cardiovascular and pulmonary disease
• renal and hepatological disease as assessed per biochemistry
• musculo-skeletal deviations limiting physical performance
• substance abuse
• other than omnivorous diet

Trial design

Primary purpose: Basic Science

Allocation: Non-Randomized

Interventional model: Crossover Assignment

Masking: None (Open label)

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60 participants in 3 patient groups

Young lean men and women

Experimental group

Description:

25 to 40 y.o. participants with body mass index in range of 18.5 to 25

Treatment:

Other: Exercise

Other: Fasting control

Young obese men and women

Experimental group

Description:

25 to 40 y.o. participants with body mass index in range of 30 to 45.

Treatment:

Other: Exercise

Other: Fasting control

Elderly men and women

Experimental group

Description:

65 to 80 y.o. participants with body mass index in range of 18.5 to 30.

Treatment:

Other: Exercise

Other: Fasting control