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Effect of Alpha-1 Antitrypsin Supplementation on Alcohol-Associated Hepatitis (EARTH)

M

Medical University Innsbruck

Status and phase

Not yet enrolling
Phase 2

Conditions

Alcohol-Associated Hepatitis

Treatments

Drug: Alfa1 Antitrypsin

Study type

Interventional

Funder types

Other

Identifiers

NCT06582329
EARTH-2024

Details and patient eligibility

About

The trial is designed as a prospective, single center, open label, randomized controlled pilot study evaluating the effect of A1AT (Alpha 1 Antitrypsin) on inflammation in patients with severe AAH (alcohol-associated hepatitis).

The objective is to evaluate the safety and the effect of intravenous A1AT on the systemic inflammation in patients with severe AAH. The objectives also include the assessment of A1AT on clinical outcomes including the incidence of adverse events (AEs) and serious adverse events (SAEs) and the cytokine.

Full description

About 5-7 % of patients with alcohol-associated chronic liver disease transform into acute on chronic liver disease (ACLF) per year. In patients with underlying alcohol-associated liver disease (ALD) and active drinking, a sudden onset of jaundice, malaise, decompensated liver disease and coagulopathy i.e. alcohol-associated hepatitis (AAH) might develop. In its severe form, AAH is associated with development of ACLF and bacterial infections and this disease exhibits a high short-term mortality of 20 to 50% within 3 months. Treatment options are limited currently, for instance, the use of corticosteroids.

Alpha-1 Antitrypsin (A1AT) acts as an anti-inflammatory protein by inhibiting the generation of pro-inflammatory cytokines. The investigators recently showed protective effects of A1AT in a pre-clinical experimental model of ALD, resulting in decreased levels of pro-inflammatory cytokines, less steatosis and hepatic injury. The investigators also have recently found that cirrhotic patients with A1AT concentrations less than 120 mg/dL had a significantly increased risk for death/liver transplantation in a cohort of 130 patients with ALD cirrhosis. This finding was not only significant, but also independent of the MELD-Na score, indicating that in ALD A1AT is not only a marker of reduced hepatic synthetic function. Further, significantly higher ferritin and lower transferrin in the cohort of patients with low A1AT also indicate more severe inflammation. An interventional analysis in an established mouse model of ALD showed that A1AT supplementation mitigated inflammation and histological changes further indicating that low AAT (Alpha 1 Antitrypsin) is a driver and not the consequence of tissue damage in ALD. These data support the use of A1AT in humans with severe AAH.

As a pilot trial the study also aims to establish important preliminary data for future studies and design of larger trials aimed at formal evaluation of the effect of A1AT on clinical endpoints.

Enrollment

16 estimated patients

Sex

All

Ages

18+ years old

Volunteers

No Healthy Volunteers

Inclusion criteria

  1. Male or female patient ≥18 years of age at time of consent.

  2. Severe AAH (Maddrey's discriminant function score ≥ 32) at screening.

  3. No ACLF or ACLF Grade 1 at screening.

  4. Daily average intake of >80 g (men)/>60 g (women) ethanol during the past 3 months (patient reported).

  5. Understands and agrees to comply with the study procedures and provides written informed consent as documented by signature.

  6. Outpatient or hospitalized patient not being on the Intensive Care Unit (ICU) at screening.

    Inclusion criterion 7 only applies to women of childbearing potential (WOCBP)

  7. Negative urine pregnancy test, not breastfeeding & agreement to use highly-effective means of contraception during the study. Allowed are sexual abstinence, vasectomized partners (˃3 months previously-vasectomy has to be confirmed by two negative semen analyses) or the consistent and correct use of an approved contraceptive method in accordance with the product label, for example: Barrier method (such as condoms, diaphragm, or cervical cap) used in conjunction with spermicide; intrauterine device; prescription hormonal contraceptive taken or administered via oral (pill), transdermal (patch), subdermal, or intramuscular (IM) route Inclusion criterion 8 only applies to male patients

  8. Male patients who are sexually active with female partners of childbearing potential must agree to use a condom with spermicide and to use one other approved method of highly effective contraception from the time of investigational product administration for at least 90 days after the dose of investigational product and must refrain from sperm donation from Screening through at least 90 days following the last dose of investigational product.

  9. Ability to speak and read German to a level which allows fully comprehending the meaning of everything that is said and written.

Exclusion criteria

  1. Uncontrolled Diabetes Mellitus type 1 or 2 (defined by HbA1c > 10%).
  2. Corticosteroid use contraindicated.
  3. Viral hepatitis, autoimmune hepatitis, HIV infection, Wilson disease, hemochromatosis, toxic liver injury, Primary Biliary Cholangitis (PBC), Primary Sclerosing Cholangitis (PSC).
  4. Participation in another interventional clinical study within 6 months prior to screening and/or during trial participation.
  5. Presence of any active malignancy (other than non-melanoma skin cancer) which required treatment within the past 12 months.
  6. Chronic kidney disease receiving dialysis.
  7. Do Not Attempt Resuscitation (DNAR) order in place.
  8. IgA deficiency (IgA level <7mg/dL) or known intolerance to A1AT.
  9. History of liver transplantation or currently listed for liver transplant.

Trial design

Primary purpose

Treatment

Allocation

Randomized

Interventional model

Parallel Assignment

Masking

None (Open label)

16 participants in 2 patient groups

standard of care
No Intervention group
Description:
Standard of care Prednisolon 40mg will be administered over 4 weeks
Standard of care + Alpha 1 Antitrypsin
Experimental group
Description:
In the experimental arm the participants will receive Prolastin 120 mg/kg bodyweight and Prednisolon 40 mg over a time period of 4 weeks
Treatment:
Drug: Alfa1 Antitrypsin

Trial contacts and locations

1

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Data sourced from clinicaltrials.gov

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