Effect of Ca2+ Ionophore on Embryonic Development and Clinical Outcome in Cases With Previous Fertilization Arrest

TopLab Company for ART Laboratories Consultation and Training

Status: Unknown

Conditions: Fertilization Arrest

Treatments: Other: Ca2+ ionophore

Study type: Interventional

Funder types: Other

Identifiers

NCT02683031

#0010010

Details and patient eligibility

About

We aimed to investigate the efficiency of chemical oocyte activation using readymade Ca2+ ionophore on cases with previously cancelled cases due to fertilization arrest at pronuclear stage i.e: cleavage failure to find out if there is any improvement in embryonic developmental stages.

Full description

Many studies reported that Ca2+ ionophore has a positive effect on embryo development in cases with complete or partial fertilization failure. However, only one preliminary report suggested a beneficial effect of Ca2+ ionophore activation in four cases with fertilization arrest.

Enrollment: 50 estimated patients

Sex: Female

Ages: 20 to 39 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Patients with previous history of complete fertilization arrest.
• ovarian stimulation with the GnRH agonist and recombinant FSH in a long protocol.

Exclusion criteria

• Female age >40 years

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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50 participants in 2 patient groups

Without Ca2+ ionophore

No Intervention group

Description:

Patients with previous incomplete ICSI cycle due to fertilization arrest were counseled to underwent a subsequent conventional ICSI cycle.

With Ca2+ ionophore

Experimental group

Description:

Patients with previous incomplete ICSI cycle due to fertilization arrest were counseled to underwent a subsequent ICSI cycle combined with artificial oocyte activation using Ca2+ ionophore.

Treatment:

Other: Ca2+ ionophore