Effect of Chorioamnionitis on Platelet Activation and Placental Vessel Among Preterm Infants by Wnt-Flt1 Signal Pathway

Guangdong Women and Children Hospital

Status: Completed

Conditions: Chorioamnionitis; Thrombocytopenia; Preterm Infant; Placenta Diseases

Treatments: Other: chorioamnionitis

Study type: Observational

Funder types: Other

Identifiers

NCT03636698

Guangdong W&C Hospital

Details and patient eligibility

About

Objective:The purpose of this study was to explore the effect and mechanism of maternal chorioamnionitis on placental microvasculature and platelet activation among preterm infants by activating Wnt-Flt1 signal pathway .

Methods:With clinical randomized controlled trial (RCT), the cases were matched with 1:1 according to gestational age and divided into 2 groups according to the placental pathology result: chorioamnionitis group and control group. (1) To observe the platelet parameter, birth weight, thrombrocytopenia and hemorrhage complication, such as intracranial hemorrhage, retinal hemorrhage, pulmonary hemorrhage and gastrointestinal hemorrhage. (2) To observe the miscrovascular density (MVD) in placenta, platelet activating factor (CD62p,CD63) and thrombopotetin (TPO) in preterrn infants.The placental MVD was assessed by immunohistochemical method. The platelet activating factors were detected by flow cytometry. TPO was detected by ELISA. (3) To observe Wnt5a, Flt1 and VEGF in placenta and fetal circulation.The measurement data were analyzed by pair t test and conditional logistic regression. Pearson correlation analysis was used for relationship.

Full description

The exclusive criteria included :(1) born to mother with no any other maternal complications; (2) no congenital abnormalities and neonatal asphyxia; (3) died or discharged within 72 hours.

Enrollment: 64 patients

Sex: All

Ages: 1+ hour old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

1. gestational age<37weeks
2. admitted to the NICU at Guangdong Women and Children Hospital, with a date of birth from June 2016 to December 2016
3. whose mothers were diagnosed chorioamnionitis by placental histopathology

Exclusion criteria

1. born to mother with no any other maternal complications
2. no congenital abnormalities and neonatal asphyxia;
3. died or discharged within 72 hours.

Trial design

64 participants in 2 patient groups

Chorioamnionitis Group

Description:

preterm infants were born to mothers with chorioamnionitis

Treatment:

Other: chorioamnionitis

Control Group

Description:

preterm infants were born to mothers without chorioamnionitis

Treatment:

Other: chorioamnionitis