Effect of Diet on Intestinal Microbiota and Obesity Markers in Adults (TORNADO)

German Institute of Human Nutrition

Status: Completed

Conditions: Blood Markers; Intestinal Microbiota

Treatments: Dietary Supplement: low meat high fibre; Dietary Supplement: high meat low fibre

Study type: Interventional

Funder types: Other

Identifiers

NCT01449383

KBBE-2007-2-2-07_WP2.4

Details and patient eligibility

About

This study aims to investigate the direct effect of high amounts of dietary fibre and high amounts of red meat in daily diet on intestinal microbiota, anthropometry and obesity markers in healthy adults.

Full description

Human gut microbiota composition and its bacterial pathways are involved in many metabolic processes, including digestion of actually indigestible food components and fat storage. Due to that it may contributes to the developement of obesity being one of the most important risk factors for many chronic diseases. Gut microbiota is under the influence of nutrition, consumption of pro- and prebiotics can promote the growth of certain bacterial strains. In a cross over dietary intervention this work will investigate the effect of defined diets on the intestinal microbiota in 20 healthy adults. The diets contain either high amounts (more than 40g/d) of dietary fibre and low red meat (less than 30g/d) or low amounts (less than 20g/d) of dietary fibre and high amounts of red meat (200g/d). Intervention periods last 3 weeks each interrupted by a 3 weeks wash out period. Examination of participants will happen at the beginning and at the end of both interventions and will contain anthropometry, blood sample, faecal sample, urine sample and saliva sample.

Enrollment: 20 patients

Sex: All

Ages: 20 to 60 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• age 20 to 60
• obtained informed consent
• willing and able to consume defined diets
• willing and able to collect all intended samples

Exclusion criteria

• prevalent chronic disease, i.e. diabetes (type 1 and 2), cardiovascular diseases, cancer
• Antibiotic treatment within the last three month before start of studies
• Prevalent gastrointestinal diseases, disorders and surgeries
• Pregnancy and breastfeeding

Trial design

Primary purpose: Prevention

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: None (Open label)

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20 participants in 2 patient groups

low meat high fibre (lmhf)

Active Comparator group

Description:

consumption of less than 30g red meat per day and at least 40g dietary fibre per day

Treatment:

Dietary Supplement: low meat high fibre

high meat low fibre (hmlf)

Active Comparator group

Description:

consumption of 200g red meat and not more than 20g dietary fibre per day

Treatment:

Dietary Supplement: high meat low fibre