Effect of Different Centrifuge Values on Saliva High Sensitive C-Reactive Protein (Hs-CRP) Levels in Individuals With Different Smoking Amounts

Izmir Katip Celebi University

Status: Enrolling

Conditions: Smoking

Treatments: Diagnostic Test: saliva obtaining

Study type: Interventional

Funder types: Other

Identifiers

NCT06561971

2024-SAEK-0029

Details and patient eligibility

About

The purpose of this study; In periodontally healthy individuals, high sensitive c-reactive protein (hs-CRP) levels of non-smokers, smokers of less than 10 cigarettes per day and smokers of more than 10 cigarettes per day were measured at 15 rpm-10 min, 10 centrifuge values, which are frequently used in saliva studies. To determine the levels in saliva samples as a result of rpm-10 min and 10 rpm 6 min centrifugations and to determine the values at which a cytokine of this molecular weight should be centrifuged and to examine the possible correlation between these values and the clinical parameters of the amount of smoking. Materials and Methods: saliva samples were collected from 90 systemically healthy individuals who were non-smokers (N, n=30), light smokers <10 cigarettes per day (L, n=30) and heavy smokers >10 cigarettes per day (H, n=20). samples were taken. Full-mouth clinical periodontal measurements, including probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI), and plaque index (PI), were also recorded. Enzyme-linked immunosorbent assay (ELISA) was used to determine hs-CRP levels in biological samples.

Enrollment: 90 estimated patients

Sex: All

Ages: 18 to 30 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Systemically healthy

• Periodontally clinical healthy

• At least 20 permanent teeth in the mouth

• No medication for continuous use

• Those who have not used antibiotics, anti-inflammatory and systemic corticosteroid drugs in the last 6 months.

• Not in pregnancy or lactation period.

• Not received periodontal treatment in the last 6 months

  • For the non-smoker group; person who does not smoke tobacco
  • For the light-smoker group; person who smokes less than 10 cigarettes a day
  • For heavy-smoker group; person who smokes more than 10 cigarettes a day

Exclusion criteria

• Any oral or systemic disease
• Regularly using a systemic medication
• During pregnancy or lactation
• Received periodontal treatment within the last 6 months.
• Those who received antibiotic, anti-inflammatory or systemic corticosteroid medication in the last 6 months
• Individual with periodontal disease

Trial design

Primary purpose: Diagnostic

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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90 participants in 3 patient groups

Non-Smokers

Experimental group

Description:

Full-mouth clinical periodontal measurements recorded and saliva obtained.

Treatment:

Diagnostic Test: saliva obtaining

Light Smokers

Experimental group

Description:

Full-mouth clinical periodontal measurements recorded and saliva obtained.

Treatment:

Diagnostic Test: saliva obtaining

Heavy Smokers

Experimental group

Description:

Full-mouth clinical periodontal measurements recorded and saliva obtained.

Treatment:

Diagnostic Test: saliva obtaining