Effect of Different Fat Enriched Meats on the Hepatic Cholesterol Synthesis

Instituto Aragones de Ciencias de la Salud

Status: Completed

Conditions: Fatty Acids; Hyperlipidemias; Lipid Metabolism Disorders; Diet, High-Fat; Cholesterol; Sterols

Treatments: Other: Fat red meat diet; Behavioral: Changes in cholesterol metabolism; Other: Lean red meat diet

Study type: Interventional

Funder types: Other

Identifiers

NCT02259153

CEICA PI13-00106;

Details and patient eligibility

About

The objective of the study is to assess the effect of two diets with different fat composition on cholesterol metabolism. The study was a randomized cross-over trial where volunteers follow two study periods with different types of meat (lean and fat red meat) separately by a ten days wash-out period. At the beginning of the study and after the study periods the following parameters are determined: anthropometric (weight, waist, circumference and body mass index), blood pressure, dietary (72-hours dietary registry) and exercise assessments and biochemical analysis (total cholesterol, triglycerides, HDL cholesterol, LDL cholesterol, apolipoprotein A1, apolipoprotein B, iron, transferring, ferritin, uric acid, glucose, HbA1c and insulin). Serum concentration of non cholesterol sterols (sitosterol, campesterol, stigmasterol, desmosterol and lanosterol) and oxysterols (24S-hydroxycholesterol, 27-hydroxycholesterol and 7α-hydroxycholestenone) were measured by High Performance Liquid Chromatography tandem Mass Spectrometry in these subjects throughout along the study in order to demonstrate the effect of different red meat on the hepatic metabolism of cholesterol.

Enrollment: 20 patients

Sex: All

Ages: 18 to 70 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Normolipemic healthy subjects defined by levels of LDL cholesterol and triglycerides below the 90th percentile adjusted for age and sex.
• Diet and stable lifestyle with controllable feeding.

Exclusion criteria

• Pregnancy or intention of pregnancy during the study.
• Lipid lowering drugs in the last 3 months.

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Single Blind

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20 participants in 2 patient groups

Lean red meat diet

Active Comparator group

Description:

Participants were given 350 g per day of lean red meat to incorporate to their usual diet for ten days.

Treatment:

Other: Lean red meat diet

Behavioral: Changes in cholesterol metabolism

Fat red meat diet

Active Comparator group

Description:

Participants were given 350 g per day of fat red meat to incorporate to their usual diet for ten days.

Treatment:

Other: Fat red meat diet

Behavioral: Changes in cholesterol metabolism