Effect of Fentanyl on Main Opioid Receptor (OPRM1) on Human Granulosa Cells.

General Hospital of Thessaloniki "George Papanikolaou"

Status: Unknown

Conditions: Stress Reaction; Opioid Use

Treatments: Drug: Fentanyl Citrate

Study type: Observational

Funder types: Other

Identifiers

NCT03248076

Opioids-OPU

Details and patient eligibility

About

Opioids is known that produce not only analgesia but also hyperalgesia through activation of central glutaminergic system-GABA. At the same time, recently it was found that the main opioid receptor (OPRM1) is present on human granulosa cells and exogenous opiates and their antagonists can influence granulosa cell vascular endothelial growth factor (VEGF) production via OPRM1, causing ovarian hyperstimulation syndrome.

This study aims to investigate if a single exposure to opioids is enough to produce activation of stress mechanism during oocyte retrieval.

Full description

The main opioid receptor (OPRM1) is present on human granulosa cells and exogenous opiates and their antagonists can influence granulosa cell vascular endothelial growth factor (VEGF) production via OPRM1, causing ovarian hyperstimulation syndrome.

This study aims to investigate if a single exposure to opioids is enough to produce activation of stress mechanism during ultrasound-guided oocyte retrieval. It will be measured the level of cortisone before and 15min after iv administration of fentanyl on blood and on oocyte fluid.

Enrollment: 30 estimated patients

Sex: Female

Ages: 18 to 32 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• American Physical Status I-III, BMI<30,

Exclusion criteria

• Heart failure
• hepatic failure, hepatitis,
• drug abuse
• receiving b blockers
• receiving b agonists (even bronchodilators)

Trial design

30 participants in 1 patient group

Fentanyl citrate

Description:

Baseline blood sample and oocyte fluid will be collected under propofol anesthesia. Fifteen minutes after administration of 1γ/kgfentanyl, it will be collected again blood sample and oocyte fluid. Cortisone and fentanyl level will be measured in all samples.

Treatment:

Drug: Fentanyl Citrate