Effect of Inhaled Molecular Hydrogen on Recovery Process After Downhill Walking on the Treadmill

Palacky University

Status: Completed

Conditions: Inflammation; Muscle Soreness; Muscle Fatigue; Oxidative Stress

Treatments: Other: Placebo; Other: Molecular hydrogen

Study type: Interventional

Funder types: Other

Identifiers

NCT05982665

FTK_2023_12

Details and patient eligibility

About

This study assesses the effect of molecular hydrogen inhalation in healthy males on muscle soreness, fatigue, oxidative stress, and inflammation following eccentric muscle contraction during downhill walking on the treadmill.

Full description

The main aim of this randomized, double-blind, placebo-controlled crossover study is to assess the effect of molecular hydrogen inhalation on recovery after downhill walking with an additional load of 15% of body mass on the treadmill with a negative slope of -15% at a speed of 6.5 km/h for 90 min. The study includes 12 participants, healthy males aged 20-35 years. The study consists of two experimental sessions, one using molecular hydrogen inhalation and the other using placebo inhalation. Randomization is performed using a computer random generator. Molecular hydrogen is colourless, odourless, and tasteless, so it cannot be distinguished from placebo by the human senses. The washout period between sessions is set at four weeks. One week before each session, participants are asked to follow diet and physical activity rules. Variables measured during the 48 h recovery are as follow: creatine kinase level, perceived muscle soreness, reactive strength index, malondialdehydes level, and interleukin-6 level. The following variables are also measured during exercise: heart rate, ventilation and gas exchange, and blood lactate level. Analysis of variance for repeated measures and Fisher's post hoc tests are used for statistical analysis. The significance level is set at 0.05.

Enrollment: 14 patients

Sex: Male

Ages: 20 to 35 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Free of major diseases.
• Completed questionnaire Sudden Cardiovascular Death in Sport: Lausanne Recommendations.
• Signed informed consent.

Exclusion criteria

• Acute conditions contraindicating laboratory testing of sports performance.
• Hypertension.
• Dyslipoproteinaemia.
• Cardiorespiratory disease.
• Pharmacotherapy affecting heart rate.
• Musculoskeletal problems.

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Double Blind

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14 participants in 2 patient groups, including a placebo group

Molecular hydrogen

Experimental group

Description:

Molecular hydrogen inhalation for 60 min at 48 h, 24 h, and 60 min pre-exercise and for 30 min post-exercise and 24 h post exercise.

Treatment:

Other: Molecular hydrogen

Placebo

Placebo Comparator group

Description:

Placebo inhalation for 60 min at 48 h, 24 h, and 60 min pre-exercise and for 30 min post-exercise and 24 h post exercise.

Treatment:

Other: Placebo