Effect of Ischemic Postconditioning on Targeting of Annexin A5 After Forearm Exercise

Radboud University Medical Center

Status

Withdrawn

Conditions

Postconditioning

Ischemia-reperfusion Injury

Cardiovascular Disease

Treatments

Procedure: repetitive short ischemia and reperfusion of non dominant fore arm after 10 minute ischemic exercise

Study type

Interventional

Funder types

Other

Identifiers

NCT00441714

IPost

Details and patient eligibility

About

To adjust our forearm model for ischemia reperfusion injury, in order to used it for assessment of ischemia reperfusion damage in elderly and patients.

To test the effect of ischemic postconditioning on ischemia reperfusion injury in healthy volunteers, using Annexin binding after repetitive handgripping.

Full description

Recently, ischemic postconditioning has been identified as a protective intervention against ischemia-reperfusion injury. In animal studies, the signalling pathway of this (impressive) protective phenomenon is very similar to ischemic preconditioning. It opens a new avenue of post-reperfusion interventions with drugs that have been shown to mimic ischemic preconditioning. Before we can study this phenomenon in our forearm ischemia-reperfusion model, we need additional validating experiments. The purpose of this study proposal is to provide these data.

Sex

Male

Ages

18 to 50 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

Age 18-50 years
No physical limitation to perform ischemic exercise
Informed consent

Exclusion criteria

Diabetes (fasting glucose > 7,0mmol/l, or random glucose >11,0mmol/l)
hyperlipidemia (random total cholesterol > 6.5 mmol/l)
Hypertension (supine SBP/DBP > 140/90 mmHg at screening)
Any cardiovascular disease
Drug abuse
Concomitant chronic use of medication
Administration of radioactivity in research setting during the last 5 years
Participation to any drug-investigation during the previous month as checked with VIP check according to CRCN standard procedures.