Effect of Metformin on Exercise-induced Lipolysis

Charles University, Czech Republic

Status: Completed

Conditions: Diabetes Mellitus, Type 2

Treatments: Drug: Metformin Oral; Other: Exercise; Drug: Metformin local in AT

Study type: Interventional

Funder types: Other

Identifiers

NCT03189732

15-03139S

Details and patient eligibility

About

The aim of this study is to investigate whether metformin has inhibitory effect on exercise-induced lipolysis in subcutaneous adipose tissue in healthy lean man. Ten healthy lean mean will follow protocols consisting of 60 minutes of exercise on bicycle ergometer combined with a) orally administered metformin and b) locally administered metformin into adipose tissue and c) without metformin treatment. Microdialysis is used for assessment of metabolites in situ in subcutaneous adipose tissue.

Full description

Metformin and physical activity are often recommended as the first therapeutic agents to improve glycemic control for prediabetes and type 2 diabetes (ADA). It has been reported that metformin decrease plasma concentration of non-esterified fatty acids in patients with type 2 diabetes which might be associated with the inhibition of lipolysis in adipose tissue. However, the inhibition of lipolysis during physical exercise, would not be probably beneficial. Thus, the effect of metformin on lipolysis during exercise is investigated in this protocol. As the lipolysis regulation is often impaired in obese and diabetic patients, the healthy lean men were chosen for this pilot study. Glycerol will be analyzed as a measure of lipolytic rate in adipose tissue and in plasma. Lactate levels will be observed. Our findings should provide a new background about the combination of physical activity and metformin medication.

Enrollment: 10 patients

Sex: Male

Ages: 25 to 45 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• healthy lean men, non obese, BMI 20-28 kg/m2

Exclusion criteria

• weight change within the 3 months before the study,
• hypertension,
• impaired fasting glucose
• diabetes
• hyperlipidemia
• drug or alcohol abuse
• smoking

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: None (Open label)

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10 participants in 3 patient groups

Metformin oral +exercise

Experimental group

Description:

Metformin, one dose 2000mg, 2.5h before the start of 60 min physical exercise

Treatment:

Other: Exercise

Drug: Metformin Oral

Metformin local in AT +exercise

Active Comparator group

Description:

Metformin perfused into microdialysis probe inserted in adipose tissue, 0.3ug/min 1.5h before the exercise and during 60min physical exercise

Treatment:

Drug: Metformin local in AT

Other: Exercise

No metformin + exercise

Active Comparator group

Description:

60min physical exercise without pretreatment of metformin

Treatment:

Other: Exercise