Effect of Oocyte Denudation Time and Intracytoplasmic Sperm Injection Time on Embryo Quality

Universitas Padjadjaran

Status: Completed

Conditions: Fertility Disorders

Treatments: Procedure: intracytoplasmic sperm injection

Study type: Observational

Funder types: Other

Identifiers

NCT05448859

OBGY-202206.02

Details and patient eligibility

About

An observational analytic retrospective study was conduct using cross-sectional study. The subject were oocytes from in-vitro fertilization procedures using the ICSI method at the assisted reproductive technology clinic in a private hospital in Bandung for the period 2017 - 2019. Three variables were oocyte denudation time, ICSI time and embryo quality collected from samples that met the research criteria.

Full description

This study analyses the different oocyte denudation time after ICSI on all embryos generated during IVF procedures between 1 Januaryr 2017 and 31 December 2019. All oocytes arer categorised on how many hours their denudation times were and these were then assessed for their qualities.

Enrollment: 200 patients

Sex: All

Ages: 3 to 8 hours old

Volunteers: No Healthy Volunteers

Inclusion criteria

• oocytes collected from in-vitro fertilisation procedures involving intracytoplasmic sperm injection

Exclusion criteria

• oocytes from patients >40 years old
• oocytes from primary infertility >5 years
• endometriosis
• polycystic ovarian syndrome

Trial design

200 participants in 5 patient groups

3-4 hours denudation

Description:

oocytes with 3-4 hours denudation time

Treatment:

Procedure: intracytoplasmic sperm injection

4-5 hours denudation

Description:

oocytes with 4-5 hours denudation time

Treatment:

Procedure: intracytoplasmic sperm injection

5-6 hours denudation time

Description:

oocytes with 5-6 hours denudation time

Treatment:

Procedure: intracytoplasmic sperm injection

6-7 hours denudation time

Description:

oocytes with 6-7 hours denudation time

Treatment:

Procedure: intracytoplasmic sperm injection

more than 7 hours denudation time

Description:

oocytes with more than 7 hours denudation time

Treatment:

Procedure: intracytoplasmic sperm injection