Effect of Partial Sleep Deprivation on Immunological System in Peripheral Blood

Hadassah Medical Center

Status: Unknown

Conditions: Sleep Deprivation

Treatments: Other: Blood withdrawal

Study type: Observational

Funder types: Other

Identifiers

NCT01930279

LDB001

Details and patient eligibility

About

Sleep deprivation was found to affect many organs including the immune system and predisposing for various health consequences including diabetes hypertension infections and increase in neoplastic diseases.

Subjects will be evaluated for immune parameters in peripheral blood test following a regular nigh sleep and compared with a test performed following a night shift in which they slept less than 3 hours.

Each participant will serve as its own control.

Full description

Overall Study Design and Plan

1. Subjects will be bled on the day of the experiment between 8and 9 AM.
2. Blood tubes will be tested for T cell markers, serum cytokines levels, and other immune parameters by western blots.
3. The first tube will be tested on the day of experiment.
4. The second tube will kept in the lab and will be tested 24 hours later.
5. The third tube will be kept in the pocket of the subject and be tested 24 hours later.
6. The fourth tube will be kept in the pocket of another subject and will be tested 24 hours later.
7. The subject will be tested again following 24 hours.
8. Each subject will be tested twice following a regular 6-8 hours of sleep, and following an on call duty in which he slept less than 3 hours.

Enrollment: 50 estimated patients

Sex: All

Ages: 18 to 40 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

Male and female ages 18-40 signed confirmed consent no evidence of chronic disease or chronic medications

Exclusion criteria

Any type of acute infection or other acute illness

Trial design

50 participants in 1 patient group

surface markers on T cells as assessed by FACS

Treatment:

Other: Blood withdrawal