Effect of Platelet Rich Plasma and Keratinocyte Suspensions on Wound Healing

Vaud University Hospital Center

Status and phase: Completed; Phase 1

Conditions: Skin Graft

Treatments: Biological: Autologous Platelet Rich Plasma; Biological: Keratinocyte suspension; Other: Standard dressings

Study type: Interventional

Funder types: Other

Identifiers

NCT00856934

132/03

Details and patient eligibility

About

This prospective randomized trial evaluated the impact of autologous keratinocytes suspended in platelet concentrates on healing skin graft donor site wounds. It was hypothesized that the treatment would speed and improve wound healing.

Enrollment: 45 patients

Sex: All

Ages: 18 to 80 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Skin graft donor sites total surface < 15% BSA
• Skin graft donor site size: 0.2mm deep, 30 to 40 cm2 surface area

Exclusion criteria

• Patients treated with immunosuppressors
• Patients treated with corticoids
• Patients suffering from terminal renal insufficiency
• Patients suffering from severe peripheral arteriopathy

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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45 participants in 3 patient groups

Control

No Intervention group

Description:

Wounds covered with standard dressings: three layers of paraffin gauze, standard bandages, elastic bandage.

Treatment:

Other: Standard dressings

PRP

Experimental group

Description:

PRP sprayed onto the wound bed with Calcium Choride. Wounds covered with same standard dressings used in control group.

Treatment:

Biological: Autologous Platelet Rich Plasma

Other: Standard dressings

PRP+K

Experimental group

Description:

Keratinocytes suspended in PRP sprayed onto the wound bed with Calcium Choride. Wounds covered with same standard dressings used in control group.

Treatment:

Biological: Autologous Platelet Rich Plasma

Other: Standard dressings

Biological: Keratinocyte suspension