Effect of Platelet Storage on Complement Activation

Shimaa Abdeleem

Status: Unknown

Conditions: Platelet Storage

Study type: Observational

Funder types: Other

Identifiers

NCT03280823

platelets and complement

Details and patient eligibility

About

Platelet units are stored and complement factors are evaluated

Full description

Ten units of platelets will be selected from the regular inventory of the Central Blood Transfusion Services/Assiut University Hospitals . All units of platelets will be collected, processed, and tested following the standard procedures.

Thesis units will be stored on a platelet rotator with the temperature controlled between 22 and 24c. Samples will be obtained using a sterile technique .

The investigators will take basal sample and samples on day 2,3,4 and 5. Complement components will be assayed : C4 (classical pathway), C3 (common pathway) and C5 (terminal pathway).

This evaluation will be done using ELISA technique

Enrollment: 10 estimated patients

Sex: All

Ages: 18 to 45 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Negative screening and confirmatory tests will be done for all platelet donors

Exclusion criteria

• Use of aspirin or non steroidal anti-inflammatory drugs or medication affecting the platelets.